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Expression of Apoptosis-Related Genes in LTED Cells: Real-Time RT-PCR Analysis

This study investigates the expression of apoptosis-related genes in LTED (long-term estrogen-deprived) MCF-7 cells. Real-time RT-PCR analysis was conducted using RNA extracted from MCF-7 cells cultured without estrogen. The methodology is based on previous research (Chan et al., 2002; Martin et al., 2003), which provides a foundation for understanding the molecular changes in cells lacking estrogenic stimulation. This analysis aims to elucidate the apoptotic mechanisms in LTED cells, potentially offering insights for breast cancer treatment strategies.

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Expression of Apoptosis-Related Genes in LTED Cells: Real-Time RT-PCR Analysis

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  1. LTED LTED LTED LTED LTED Supplementary Figure 5. Expression of apoptosis related genes in LTED Cells Real-time RT-PCR analysis was performed using RNA prepared from MCF-7 cells cultured in the absence of estrogen, as described previously (Chan et al. 2002 J Steroid Biochem & Mol Biol 81: 333-341; Martin et al. 2003 J BiolChem 278: 30458-30468).

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