1 / 9

SLBP

Choe _ Fig S1. A. B. After IP ( a -FLAG). His-CTIF (365-598). Before IP. CTIF. CTIF. pcDNA3-FLAG. SLBP(128-270). SLBP(128-270). SLBP(1-127). SLBP(1-127). GST-SLBP(1-127). GST-SLBP(1-127). pCMV-Myc. GST- Trx. GST- Trx. SLBP. SLBP. SLBP. SLBP. GST. GST. kDa. 40.

gurit
Télécharger la présentation

SLBP

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Choe_Fig S1 A B After IP (a-FLAG) His-CTIF (365-598) Before IP CTIF CTIF pcDNA3-FLAG SLBP(128-270) SLBP(128-270) SLBP(1-127) SLBP(1-127) GST-SLBP(1-127) GST-SLBP(1-127) pCMV-Myc GST-Trx GST-Trx SLBP SLBP SLBP SLBP GST GST kDa 40 GST-SLBP (1-127) FLAG-CTIF GST-Trx Myc-SLBP 35 GST 25 Myc-SLBP(128-270) His-CTIF (365-598) Myc-SLBP(1-127) Input GST pull-down CBP80 b-actin Merge DAPI VFP C VN VC D SLBP(WT)-VN CTIF-VC E SLBP(W75A)-VN CTIF-VC

  2. Choe_Fig S2 A Control CTIF siRNA 10 20 30 40 10 20 30 40 Min past HU 0 0 HIST2H2AA mRNA SLBP(WT) SLBP(W75A) pcDNA3-FLAG Min past HU H3F3A mRNA FLAG-SLBP(WT or W75A) 95 3 69 9 49 6 33 0 100 HIST2H2AA mRNA / H3F3A mRNA (%) -actin 96 7 62 9 908 718 100 B 10 10 20 20 30 30 40 40 0 0 160 140 120 100 HIST2H2AA mRNA /H3F3A mRNA (%) 80 C 60 Control siRNA 40 20 0 hr 4hr 6hr 8hr 10hr 250 250 250 250 250 0 Min past HU 0 10 20 30 40 0 10 20 30 40 Control CTIF siRNA 2N 4N 2N 4N 2N 4N 2N 4N 2N 4N CTIF siRNA 0 hr 4hr 6hr 8hr 10hr 250 250 250 250 250 2N 4N 2N 4N 2N 4N 2N 4N 2N 4N D

  3. Choe_Fig S3 A B Control CTIF siRNA + + Control CTIF siRNA HU + + HU Uridylated HIST2H2AA mRNA CTIF H3F3A mRNA b-actin 26954 Uridylated HIST2H2AA mRNA / H3F3A mRNA (%) 100 11833 100

  4. Choe_Fig S4 A SLBP(WT) pcDNA3-FLAG Min past HU 10 20 30 40 10 20 30 40 0 0 FLAG-SLBP(WT) -actin B SLBP(WT) pcDNA3-FLAG Min past HU 10 20 30 40 10 20 30 40 0 0 HIST2H2AA mRNA H3F3A mRNA 89 5 67 2 43 2 34 2 100 HIST2H2AA mRNA / H3F3A mRNA (%) 90 3 36 6 725 508 100

  5. Choe_Fig S5 A eIF4E-BP1 pCMV-Myc + + HU Myc-eIF4E-BP1 -actin B C 120 120 100 100 80 80 Relative RLuc activity / RLuc mRNA (%) RLuc mRNA / SMG7 mRNA (%) 60 60 40 40 20 20 0 0 + + + + HU HU pCMV-Myc eIF4E-BP1 pCMV-Myc eIF4E-BP1 D E 120 120 100 100 HIST1H1C mRNA / H3F3A mRNA (%) HIST2H2AA mRNA / H3F3A mRNA (%) 80 80 60 60 40 40 20 20 0 0 + + + + HU HU pCMV-Myc eIF4E-BP1 pCMV-Myc eIF4E-BP1

  6. Choe_Fig S6 A B 160 140 120 100 eIF4E-T Dicistronic mRNA / SMG7 mRNA (%) pCMV-Myc 80 + + 60 HU 40 Myc-eIF4E-T 20 -actin 0 + + HU pCMV-Myc eIF4E-T C D 5 1.5 4 1 Relative FLuc activity / dicistronic mRNA Relative RLuc activity / dicistronic mRNA 3 2 0.5 1 0 0 + + + + HU HU pCMV-Myc eIF4E-T pCMV-Myc eIF4E-T E F 120 120 100 100 80 80 HIST1H1C mRNA / H3F3A mRNA (%) HIST2H2AA mRNA / H3F3A mRNA (%) 60 60 40 40 20 20 0 0 + + + + HU HU pCMV-Myc eIF4E-T pCMV-Myc eIF4E-T

  7. Choe_Fig S7 A B CBP80/20-dependent translation eIF4E-dependent translation Stop Stop eIF4GI/II eIF3 Cap eIF4E AUG SLIP1 CTIF Histone mRNA decay 80 60S eIF3 Genotoxic stress Cap AUG 20 40S 40S 20 3’-5’ decay CTIF SLBP SLBP Stop Stop SLBP eIF3 SLIP1 SLIP1 eIF4GI/II eIF3 AUG Cap eIF4E AUG 80 Cap 5’-3’ decay Dcp1/2

  8. Choe_Fig S8 FLAG-SLBP + Cross-link a-FLAG a-FLAG IP mIgG mIgG FLAG-SLBP CTIF CBP80 eIF3b eIF4GI eIF4E b-actin

  9. Choe_Fig S9 Myc-SLIP1 After IP (a-FLAG) Before IP + + RNase A SLBP(W75A) SLBP(W75A) SLBP(W75A) SLBP(W75A) SLBP(WT) SLBP(WT) SLBP(WT) SLBP(WT) pcDNA3-FLAG FLAG-SLBP CTIF Myc-SLIP1 eIF4GI eIF4E b-actin

More Related