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Antigenicity

Antigenicity. Requirements to be an immunogen Low mw antigens (haptens) Adjuvants Primary vs secondary responses. Concept of a Hapten. r/c = Kn-Kr. c = concentration of free antigen (hapten) K = K a = equilibrium constant r = hapten bound per unit Ab n = Valence of Ab. Polyclonal Ab.

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Antigenicity

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  1. Antigenicity • Requirements to be an immunogen • Low mw antigens (haptens) • Adjuvants • Primary vs secondary responses

  2. Concept of a Hapten

  3. r/c = Kn-Kr c = concentration of free antigen (hapten) K = Ka = equilibrium constant r = hapten bound per unit Ab n = Valence of Ab

  4. Polyclonal Ab

  5. 1/c = K(2) – K(1) 1/c = K0

  6. Factors which influence Ab affinity • Temp, pH, ionic strength • Polyclonal vs monoclonal • Ab class • Type of antigen

  7. Antibody Combining Site Location

  8. Figure 3-4

  9. Figure 3-1

  10. Evidence that Hv interacts with Ag 3 lines of evidence

  11. Affinity Labeling

  12. Comparison of Hv sequences of monoclonals binding the Same vs binding a different antigen epitope

  13. All interactions are non-covalent

  14. Affinity vs avidity – depends on valence

  15. IgM is the most avid of Ab classes

  16. Composition of immune ppts at different Ab/Ag ratios

  17. Gel Diffusion Analysis to determine relatedness of antigens

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