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Dengue

Detection of Dengue Virus RNA in Blood Donors from Honduras and Brazil with a Prototype Transcription-Mediated Amplification Assay. Dengue. Over 2.5 billion people live in risk areas for dengue infection

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Dengue

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  1. Detection of Dengue Virus RNA in Blood Donors from Honduras and Brazil with a Prototype Transcription-Mediated Amplification Assay

  2. Dengue • Over 2.5 billion people live in risk areas for dengue infection • WHO estimates that in 2004 there were 50-100 million cases of DF, 500,000 cases of dengue hemorrhagic shock syndrome (DHS) and 20,000 consequent deaths • Epidemic is expanding due to global warming, expanding vectors and trafficking of subtypes • Dengue the most important arboviral disease of humans

  3. Expanding global epidemic of Dengue

  4. Number of dengue fever cases reported per month, Brazil. A) 1986–1993, B) 1994–2003. Dark bars represent January.

  5. Curvas epidémicas del 2002, 2003,2004 Y 2005* Casos Sospechosos de Dengue Clásico Honduras , *Semana 41 2002 (32,269 casos) 2003 (16,559 casos) 2004 (19,971 casos) 2005 (16,037 casos)* * AÑO EPIDÉMICO 1995 EXCLUÍDO FUENTE: Telegrama Epidemiológico.

  6. Analytical sensitivity of the prototype dengue TMA assay • DEN 1, 2, 3 and 4 diluted to 1 PFU/mL

  7. Sources of Donor Plasma from Dengue Endemic Regions 12 IR, 9 RR 11 confirmed 8 IR, 3 IR confirmation pending in progress

  8. Dengue TMA Results from Honduran Blood Donors(n = 2994)

  9. Planned studies of TT-Dengue • Transmission study in Honduras with BSF and Gen-Probe funding • Transmission study in Brazil under REDS-II funding • Collaborations with Australia, Hong Kong and Singapore blood banks

  10. Dengue Transmission Study Approach • It is mandatory in Brazil to save donor serum samples for 6 months after the transfusion • Phase 1: Prevalence Study • Dengue TMA on 10,000 unlinked donor samples to determine prevalence • Phase 2: Transmission Study • collect recipient samples ~1 week after transfusion. • capture donor samples in routine repository. • Dengue TMA on both samples. • sequence virus to confirm linkage

  11. Acknowledgements J Linnen, A Broulik, C Collins, DP Kolk, C Giachetti, L Mimms Gen-Probe Incorporated, San Diego, CA; E Vinelli Honduran Red Cross, Tegucigalpa, Honduras; E Sabino, Blood Center of Sao Paulo, Sao Paulo, Brazil; R Lanciotti, CDC, Fort Collins, CO; C Hyland, Australian Red Cross, Brisbane, Australia; L Tobler, MP BuschBlood Systems Research Institute, San Francisco, CA;

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