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Exercise 14. Plasmid Isolation and Restriction Enzyme digest. 1 2 3 4. 1 : Uncut plasmid DNA 2 : DraI digested ( Master mix D) 3 : EcoRI digested ( Master mix E) 4 : 1Kb DNA ladder. Dr. H. Park. Amp R. Cml R. pCSLA1. EcoRI.

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  1. Exercise 14. Plasmid Isolation and Restriction Enzyme digest 1 2 3 4 1 : Uncut plasmid DNA 2 : DraI digested ( Master mix D) 3 : EcoRI digested ( Master mix E) 4 : 1Kb DNA ladder Dr. H. Park

  2. AmpR CmlR pCSLA1 EcoRI ~800 bp insert EcoRI There are multiple DraI sites on the plasmid Plasmid itself is about 6.4 kb in length (without any insert) With insert here of 0.8 kb, it should be around 7.2 kb.

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