Bioprocessing Biotechniques Peer reviewed

1. Research Article Volume 10:1,2020 Journal of Bioprocessing & Biotechniques ISSN: 2155-9821 Open Access Effect of blending ratio of pineapple on sensory and physicochemical property of mango juice Yassin Hassen1,, Derese Tamiru 1, , Belay Haftu1 1 Institute of Pharmacy and Medical Science, Hebron University, Hebron, Palestine 2 Institute of Health Professions, AN-Najah University, Nablus, Palestine 3Euro Lab for Medical Analysis, Ramallah, Palestine Abstract Quality and alimental contents of single fruit juice can be ameliorated through mixing or blending process with other fruit juices. Pineapple and mango are the most popular tropical fruits with good characteristic taste. Fruit juice were developed from mango and pineapple with different proportions .The physico-chemical properties (TSS, pH and TA) and sensory quality (color , taste, Viscosity, mouth feel, aroma and overall acceptability) of developed juices were evaluated .Color properties of pineapple and mango juice blends at ratio of 85M: 15P; 70M: 30P; 55M: 45P was evaluated. Blends ratio 55M:45P juice give the best color performance interms of hue, chroma and ΔE. Physicochemical properties of juice blends ratio of 55M:55P also give results of pH (3.56) titratable acidity (0.79%), total soluble solid (14.60). Based the present study this study blending ratio of 55M: 45P resulted with best quality of juice compare to others blending ratios. Keywords: Keywords: pineapple, sensory, physicochemical, mango juice East with different locals and expatriates. Therefore, when considering the effects of ambient charges in a duopolistic market, they might be an effective policy instrument not only. The study was conducted in the Euro lab medical center in which all samples were referred by different outpatient clinics for further genetic analysis during the time period between January 2013 and January 2014. The study samples were composed of 112 unhealthy persons (63 males, 49 females) who were sending to Euro lab for further genetic investigations concerning HLA. About 2 mL aliquots of peripheral blood samples of these patients were collected and stored in EDTA tubes. DNA was extracted from a 200 μl peripheral blood then; DNA amount and DNA purity were quantified for each DNA sample by Nano-drop. Genotyping of polymorphisms of AS patients a sharp decrease in death phase at 60 minutes but the organism survived at 180 minutes. It was observed that A1 LexA shows a rapid decrease in activity from 0 to 180 minutes and controls were performed by real time polymerase chain reaction chain reaction system. Simple statistical methods (median, frequency and percentage) where used. Introduction Figure 1: Figure 1: Mechanism of Action of Isolated Compound A1(Epigallocatechin, Epicatechin and Stigmasterol phytosterol (Synergy) By Gene Expression (Repressor LexA) on Bacillus subtilis. Studies indicate that appropriate DRR interventions can be implemented only when the vulnerability of people to a particular hazard has been assessed [12,13] hence the necessity of our study. Preparedness for recovery through developing recovery policies, and strengthening or establishing institutional responsibilities at national and local levels is also an essential part of DRR [14-18]. When assessing flood vulnerability or resilience due caution has to be exercised since it is important to understand that resilience and vulnerability are not necessarily opposite ends of a spectrum for individuals or communities (Figure 1).The capacity of communities to absorb external shocks due to social, political or environmental changes is a function of three. Literature review Patients and Methods Published studies of HLA-B27 were reviewed in Arab patients. We divided Arab populations into three parts: The research was carried out in two stages: The laboratory and The literature review A) Levant which includes Jordan, Syria, Lebanon, and Palestine. B) Arabian Peninsula which includes Saudi Arabia, UAE, Kuwait, Qatar. C) North Africa which includes Egypt and Morocco. Laboratory We looked up all the articles from PubMed and Medline addressing HLA- B27 prevalence among all these areas on AS patients and healthy A retrospective review of one hundred nineteen patients with symptoms of AS were referred for HLA-B27 genetic testing during the time period between January 2013 and January 2014. Palestine is an Arab country, in the Middle *Address for Correspondence: Address for Correspondence: Yassin Hassen, , Hawassa University School of Nutrition, Food Science and Technology, ethiopia, Tel: +970598401095; Fax: +97022229303; E-mail : yassin.hassen@gmail.com Copyright: Copyright: © 2020 Yassin H, et al. This is an open-access article distributed under the terms of the creative commons attribution license which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. Received: Received: 11 December 2020; Accepted: Accepted: 27 February, 2020; Published: ; Published: 02 March, 2020

2. Hassen et al. J Bioprocess Biotech, Volume 10: 1, 2020 individuals. We also searched for large studies conducted on western populations over the last couple of years for comparison. In contrast, the percentage of HLA-B27 among Emirian Arabs is extremely low (0.5%) [5]. In Saudi Arabia, HLA-B27 was checked in 12/15 Arabs, locals comprised 6/15, and the percentage was only 67% [7]. In Kuwait, the percentage was higher (77.8%), but in Kuwaitis were only 9 out of 58 [23]. Results In our study the percentage in 18 Palestinian patients 10 (43.4%) male, 8 (34.7%) female, which was slightly lower than the findings in 2 Jordanian papers, 75% in the first paper with total of 20 patients tested and 81% in the second paper with total of 52 patients tested [17,19]. It represented as +ve HLA-B27 distribution among patient with AS in Palestine in which a total of 112 cases and 39 controls of Palestinian populations were typed for HLA-B27 using PCR-SSP. Of all the individuals (112), 23 were HLA-B27 positive (20.5%) in Palestine. The result of the analysis is presented in Tables 1-3. Conclusion Table 1: Table 1: Comparison of HLA-B27 among ankylosing spondylitis patients in Palestine and Arabs population. This is the first study to evaluate the prevalence of HLA-B27 in Palestine and in unhealthy patient with AS also living in Palestine. Results suggest the prevalence of HLA-B27 among Palestinian patients is low and with good agreement the prevalence in Lebanon 23.6% and Egypt 29.3% but the findings is in contrast to Jordan 71%, Saudi Arabia 67% and Kuwait 25.7%. Percentage Percentage of + ve of + ve Number Number Number Number health health of of There is a lack of knowledge about the percentage of HLA-B27 in healthy Palestinian. However, we have a huge lack of data among Arabs in different regions in addition to that; the studies conducted are few with small number of subjects. No data was found related to Libya, Yemen or Sudan. Further studies with larger number of patients and studies that look at the prevalence of AS in Palestine and at the prevalence of HLA-B27 in healthy Palestinian are needed. More wide based population studies are needed to prevent delays in diagnosis and to start the treatment earlier for better disease outcome. HLA-B27 HLA-B27 Country Country of AS of AS References References antigen antigen Individual Individual patient patient among AS among AS Patient (%) Patient (%) Palestine 23 20.50 1 89 Jordan 70 71.00 2 2579 Syria 50 60.00 3 217 Conflict of Interest Lebanon 16 23.60 4 N/A The authors declared no conflicts of interest with respect to the authorship and/or publication of this article. Kuwait 35 25.70 8 544 Qatar 66 74.00 9 N/A Financial Disclosure UAE 16 56.00 10 N/A The authors received no financial support for the research and/or authorship of this article. Discussion References Ankylosing spondylitis, is known to be highly heritable, with >90% of the risk of developing the disease determined genetically [13,14]. Genetic factors have been strongly implicated in its etiology [2]. Despite the prominence of HLA-B27 in genetic susceptibility of AS, its contribution to overall genetic predisposition is 16% to 40%. 1. Smith, John. “Studies in Pop Rocks and Coke.” Weird Science 12 (2009): 78-93. 2. Smith, John, and Jane Doe. “Studies in Pop Rocks and Coke.” WeirdScience 12 (2009): 78-93. 3. Smith, John, Austin Kaufmann, Jennifer Monroe, and Jane Doe.“Studies in Pop Rocks and Coke.” Weird Science 12 (2009): 78-93. Human leucocyte antigen-B27 is a MHC class I molecule that is encoded on chromosome 6p. There is a wide range of variation in the frequency of HLA-B27 allele and the distribution of its subtypes across populations which has a significant impact on the prevalence of AS in different racial/ethnic groups [15]. 4. Grazer, Brian, and Charles Fishman. A Curious Mind: The Secret toa Bigger Life. New York: Simon & Schuster, USA, 2015. 5. Farhad, Manjoo. “Snap Makes a Bet on the Cultural Supremacy of the Camera.” New York Times, March 8, 2017. To our knowledge this is the first study to investigate HLA-B27 in a large number of different genetic traits of Palestinian population. The study was conducted to assess the prevalence of HLA-B27 among unhealthy patients with AS in Palestine. The prevalence in Palestinian is lower than the prevalence reported in Jordan of 75% and 81% and in Iraq of 84% [16-18]. 6. Michiko, Kakutani. “Friendship Takes a Path that Diverges.” Review of Swing Time, by Zadie Smith, New York Times, November 7,2016. 7. Cynthia, Lillian Rutz. “King Lear and its Folktale Analogues.” PhD Diss., University of Chicago, (2013): 99–100. However, in a study done in Turkey there was no association found between AS and IL23R rs11209032 and rs1004819 polymorphisms in this Turkish AS cohort [19-21]. Data from other Arabian Gulf countries showed that the prevalence of HLA-B27 is lower as in UAE, a study found the percentage was 56% among Arabs and none of them were locals in addition to the study sample was small (28 pts.) [22]. 8. Smith, John. “Studies in Pop Rocks and Coke.” Weird Science 12 (2009): 78-93. 9. Smith, John, and Jane Doe. “Studies in Pop Rocks and Coke.” Weird Science 12 (2009): 78-93. 10. Smith, John, Austin Kaufmann, Jennifer Monroe, and Jane Doe.“Studies in Pop Rocks and Coke.” Weird Science 12 (2009): 78-93. Page 2 of 3

3. Hassen et al. J Bioprocess Biotech, Volume 10: 1, 2020 11. Grazer, Brian, and Charles Fishman. A Curious Mind: The Secret to a Bigger Life. New York: Simon & Schuster, USA, 2015. 12. Farhad, Manjoo. “Snap Makes a Bet on the Cultural Supremacy of the Camera.” New York Times, March 8, 2017. 13. Michiko, Kakutani. “Friendship Takes a Path that Diverges.” Review of Swing Time, by Zadie Smith, New York Times, November 7,2016. 14. Cynthia, Lillian Rutz. “King Lear and its Folktale Analogues.” PhD Diss., University of Chicago, (2013): 99–100. How to cite this article: Yassin Hassen, Tamiru Derese, Haftu Belay. "Effect of blending ratio of pineapple on sensory and physicochemical property of mango juice ". J Bioprocess Biotech10 (2020). Page 3 of 3