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IP: HA Myc HA PowerPoint Presentation
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IP: HA Myc HA

IP: HA Myc HA

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IP: HA Myc HA

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  1. A B Figure S1. Knock down of FSP27 increases while over-expression of FSP27 decreases the release of FFA from human adipocytes. FA were measured after 2.5 h incubation of cells in KRB with 4% BSA. All values are means ± SD; p value was estimated by unpaired t-test. B A Egr1 positive control Egr1 positive control IP: Myc HA Myc IP: HA Myc HA Egr1 Egr1 Myc HA HA-FSP27: + + - Myc-FSP27: + + - Egr1: + + - Egr1: + + - Figure S2. FSP27 co-immunoprecipitates with both HA- and Myc-tagged Egr1. HEK293T cells growing in 65-mm dishes were transfected with 4 µg of HA-tagged FSP27 (panel A) or Myc-tagged FSP27 (panel B) cDNA and 2 µg of Egr1 cDNA. Cell lysates were collected 24 h post-transfection and immunoprecipitated with 2 µg of either HA or Myc antibody and Protein A/G magnetic beads. The eluates were then separated by 12.5% PAGE and immunoblotted using HA, Myc, and Egr1 antibodies as indicated.