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HIM-3 Protein in C. elegans: Role in Synaptonemal Complex Formation and Male Incidence

The him-3 gene in C. elegans encodes a homolog of the S. cerevisiae HOP1 protein, critical for synaptonemal complex formation during meiosis. With a 1.2 kb mRNA sequence encompassing four exons and three introns, it is located on chromosome IV (positions 6,977,403 to 6,978,631). Mutants exhibit a high incidence of males and increased X chromosome loss, resulting in embryonic lethality. The HORMA domain in the HIM-3 protein suggests a mechanistic link between early prophase events of synapsis, recombination, and later segregation processes.

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HIM-3 Protein in C. elegans: Role in Synaptonemal Complex Formation and Male Incidence

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  1. him-3 High Incident Males (increased X chromosome loss) By Chang Lee

  2. him-3 encodes a homolog of the S. cerevisiae HOP1 protein which is required for the formation of the synaptonemal complex during meiosis Predicted by GeneFinder Washington University, St. Louis MO.

  3. How large? 1.2 kb mRNA sequence : 1113 bp 4 exons The gene contains 3 introns

  4. What chromosome is the gene on? chromosome IV positions 6,977,403 to 6,978,631

  5. There are available cDNA CK575867, EB994223, OSTF002C4_1 EC023874 CK589379 AF172351 EST data

  6. Mutants Mutant e1147 gk149 vv6 e1256 me80 Its phenotype is embryonic lethal, partial, high incidence of males, increased X chromosome loss

  7. The final gene product is a HOP1-like protein him-3 encodes a homolog of the S. cerevisiae HOP1 protein

  8. homologs in other species C. Briggsae 4.5e-66 C remanei 1.4e-27 C Elegans 2e-22 M Musculus 3.6e-12 H sapiens 4.8-12

  9. structural components The DNA-binding HORMA motif is seen in the product of this gene The HORMA (for Hop1p, Rev7p and MAD2) domain has been suggested

  10. molecular function HIM-3 may be part of a mechanistic link that exists between the early prophase events of synapsis and recombination, and later events such as segregation

  11. references http://www.genesdev.org/cgi/search?session_query_ref=rbs.queryref_1209325456816&COLLECTIONS=hw1&JC=genesdev&FULLTEXT=%28size+AND+of+AND+him-3+AND+gene%29&FULLTEXTFIELD=lemcontent&RESOURCETYPE=HWCIT&ABSTRACTFIELD=lemhwcompabstract&TITLEFIELD=lemhwcomptitle http://www.wormbase.org/db/gene/gene?name=WBGene00001862;class=Gene http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?db=worm&q=4H743 http://linkinghub.elsevier.com/retrieve/pii/S0960982204002179 http://www.sanger.ac.uk/cgi-bin/Pfam/getacc?PF02301

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