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Peri and Post Operative Care of Mice

Peri and Post Operative Care of Mice. Surgical Prep in Mice. Mice are susceptible to infections and therefore aseptic technique should be followed. Pick your anesthesia. Weigh your mouse. Sterilize your instruments. Prepare the incision site. Wear gloves. Surgical Prep in Mice.

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Peri and Post Operative Care of Mice

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  1. Peri and Post Operative Care of Mice

  2. Surgical Prep in Mice • Mice are susceptible to infections and therefore aseptic technique should be followed. • Pick your anesthesia. • Weigh your mouse. • Sterilize your instruments. • Prepare the incision site. • Wear gloves.

  3. Surgical Prep in Mice • Mice have large surface area to body volume and high metabolic rate • They are susceptible to dehydration, hypothermia, and hypoglycemia. • They do not vomit.

  4. Surgical Prep in Mice • To reduce hypothermia, can do surgery on heating pad or under a heat lamp. • Also, inject mouse with warmed lactated Ringer’s which also helps with dehydration. • Do not withhold food due to inability to vomit and hypoglycemic tendencies.

  5. Surgery in Mice • Check depth of anesthesia. • Toe pinch. • Watch respiratory rate. • Watch color in ears, paws tail and nose. • Chest compressions or doxapram. • Watch cardiovascular function. • Cauterization or just holding off of cut vessels. • Body temp. • Fluids. • Close incision.

  6. Post Op in Mice • Do not leave until mouse is ambulatory. • Keep on heating pad and/or wrapped up for warmth. • Give analgesia for at least 24 hours. • Supplement food and give more fluids as necessary. • Soft bedding recommended.

  7. More info: • Free course on ALL: Working with the Laboratory Mouse. • Free CD from NIH: Training in Basic Biomethodology for Laboratory Mice.

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