1 / 10

Exploring Plant Diversity Through Genomics: The SOL Initiative

The SOL Genomics Network, established in 2003, aims to unravel how a common set of genes can lead to diverse organisms and how this knowledge can be used to sustainably meet societal needs. With more than 3,000 species in the Solanaceae family, significant evolutionary developments in South America have shaped their diversity. The network focuses on sequencing the tomato genome, developing genetic maps, and enhancing plant breeding through natural diversity. SOL's mission includes educating the public on biodiversity and creating an extensive bioinformatics platform for research.

illana-christensen
Télécharger la présentation

Exploring Plant Diversity Through Genomics: The SOL Initiative

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. SOL Genomics Network • Formed in 2003 to answer two questions: • How can a common set of genes give rise to such a wide range of morphologically and ecologically distinct organisms that occupy our planet? • How can a deeper understanding of the genetic basis of plant diversity be harnessed to better meet the needs of society in an environmentally-friendly and sustainable manner?

  2. Lynn Bohs More than 3,000 Solanaceaespecies world-wide. Major evolutionary progress in South America unaltered by ice-ages and fueled by extreme climates.

  3. 2n=2x=24 950 Mb 2n=2x=24 3000 Mb 2n=2x=24 950 Mb 2n=2x=24 1300 Mb 2n=4x=24 1800 Mb 2n=2x= 22 ca. 1000 Mb

  4. SOL Tomato Sequencing • Genome size of 950 Mb. ~35,000 genes. • 25% DNA lies in gene-dense euchromatin regions. • Anchor BACs to genetic map (S. esculentum x S. pennellii F2) then choose new BACs based on sequenced ends. Verify physical location by FISH.

  5. Comparable Maps • Arabidopsis: • Arabidopsis COSII • Eggplant: • Eggplant-LXM 2002 • Pepper: • Pepper-FA03 • Pepper-AC99 • Potato: • Potato-TXB 1992 • Tobacco: • Tobacco SSR 2007 • Tomato: • Tomato FISH map • Tomato-EXPIMP 2001 • Tomato-EXHIR 1997 • Tomato-EXPIMP 2008 • Tomato-EXPEN 1992 • Tomato-EXPEN 2000 • Tomato IL map (2) • Tomato physical map • Tomato FPC map • Tomato AGP map

  6. Chrom. 1 2 3 4 5 6 7 8 9 10 11 12 Mb* 24 26 26 19 11 20 27 17 16 10 13 11 T=220 Short arm Centromere Italy USA USA Long arm The Netherlands USA Spain USA France UK China Korea USA euchromatin euchromatin heterochromatin heterochromatin AT-rich satellite DNA AT-rich satellite DNA * Euchromatinportion

  7. The 10 missions of SOL • Sequence the reference tomato genome using BAC minimal tiling paths. • Develop deep EST databases and shotgun genomic sequences for other Solanaceae crops as well as SNP analysis of diverse germplasm. • Construct COS maps and interspecific introgression resources for all SOL crops • Establish asaturation set of mutagenesis lines and cloned gene specific tags (GST). • Construct a comprehensive phylogenetic and geographical distributional information network. • Apply transcription, proteomic & metabolic profiling to explore chemical and developmental evolution. • Apply non-destructive ‘Real Time’ physiology and phenotyping. • Improve the efficiency of plant breeding -- especially through use of natural diversity. • Educate the public about the value of biodiversity, domestication and plant improvement. • Develop an international bioinformatics platform which will facilitate a systems approach to SOL research.

More Related