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Analysis of eRF3b and eRF3a Variants in Wild Type and Mutant Constructs

This study investigates the expressions of wild type eRF3b and its mutants (F66A/F75A) alongside eRF3a in cellular contexts. Using Western Blotting (WB), we quantify protein levels tagged with Myc (5xMyc) for eRF3b and eRF3a. We aim to compare relative protein quantification against GAPDH as a loading control, examining molecular weights around 89 kDa for eRF3 proteins. The results provide insight into how mutations affect expression and functionality of these release factors in cellular environments.

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Analysis of eRF3b and eRF3a Variants in Wild Type and Mutant Constructs

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  1. 1 2 3 4 5 6 7 Supplementary Figure 1 B A eRF3b wild type eRF3b F66A/F75A eRF3b F66A eRF3b F75A pCMV-5xMyc- pCMV-5xMyc- eRF3b eRF3a eRF3a (kDa) (kDa) 117 5xMyc-eRF3b 5xMyc-eRF3b 117 (WB: -Myc) 89 5xMyc-eRF3a 89 (WB: -Myc) 89 Cellular eRF3 (WB: -eRF3) 117 5xMyc-eRF3a GAPDH 35 Cellular eRF3 89 (WB: -GAPDH) (WB: -eRF3) 1 2 3 4 5

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