1 / 18

Cytotoxic and Immunologic Effects of Silver Nanoparticles

Cytotoxic and Immunologic Effects of Silver Nanoparticles. Shaun Cote. What are Nanoparticles?. Any substance that has one dimension of 1-100 nanometers Commonly used nanoparticles include colloidal gold and titanium dioxide (TiO 2 ). Nano Scale. Why is this important?.

indira-bryant
Télécharger la présentation

Cytotoxic and Immunologic Effects of Silver Nanoparticles

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Cytotoxic and Immunologic Effects of Silver Nanoparticles Shaun Cote

  2. What are Nanoparticles? • Any substance that has one dimension of 1-100 nanometers • Commonly used nanoparticles include colloidal gold and titanium dioxide (TiO2)

  3. Nano Scale

  4. Why is this important? • “The biokinetics of NSPs are different from larger particles. When inhaled, they are efficiently deposited in all regions of the respiratory tract; they evade specific defense mechanisms; and they can translocate out of the respiratory tract via different pathways and mechanisms (endocytosis and transcytosis).” Nanotoxicology: An Emerging Discipline Evolving from Studies of Ultrafine Particles. Environmental Health Perspectives, Oberdörster et al. 113(7), July 2005.

  5. Downsides to Silver Nanoparticles • Possible negative effects due to the chemistry of small molecules

  6. How they are Made • Metal salt solutions are boiled and then reduced via a reducing agent

  7. Silver Nanoparticles in the Market • Samsung • Washing machine, refrigerator and air conditioners • Nanohorizons • Smart Silver – silver nanoparticles intertwined with fabric to repel odor and microbes • Aigon • SilverClene anti-microbial cleaning solution

  8. Project Goals • To explore the effects of silver nanoparticles by testing the: • Cytotoxicity on established cell lines: • Production of specific immune molecules

  9. Testing • MTT Assay – tests for metabolic activity • TNF-α ELISA – Antibody test for TNF-α secretion from cells • RT-PCR – Measure of RNA activity

  10. Cell Lines Tested • RAW 264.7 – Mouse macrophage • Jurkat – Human T cells • WHTBF-6 – Human lung fibroblast

  11. MTT Assay • Cells are cultured, then treated with silver nanoparticles • MTT is added, and blue crystals are formed if the cells are viable • Correlation between concentration of nanoparticles and viability of the cells

  12. MTT Assay - Results

  13. TNF-α ELISA • Test based on antibody capture of TNF-α, a protein molecule essential in triggering an immune response • Measured OD of sample wells compared to a standard curve

  14. TNF-α ELISA Results • Concentration of silver nanoparticles versus relative concentration of TNF-α

  15. RT-PCR • A method of amplifying target RNA to determine how silver nanoparticles affect gene expression of specific cytokines

  16. Results of RT-PCR • Only tested GAPDH, a housekeeping gene • Further tests include the cytokines TNF-α, IL-6, and IL-1β

  17. Further Studies • Continuing RT-PCR studies on other cytokines

  18. Acknowledgements • Dr. Ah-Kau Ng and Lab • Theresa Knight • Jennifer Walker • SURF program

More Related