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Enhancing Antibiotic Delivery Using Protein Nanoparticles Against Agrobacterium Tumefaciens

This study explores the use of antibiotic-loaded protein nanoparticles to improve antibacterial efficacy against Agrobacterium tumefaciens. Traditional pesticides often fail to reach their targets effectively, leading to environmental issues. This research investigates the effectiveness of albumin nanodroplets versus nanofibers in delivering antibiotics in vitro and in vivo. We hypothesize that these two drug delivery methods will demonstrate comparable results in terms of antibiotic release and bacterial inhibition, potentially reducing side effects and improving therapeutic outcomes while minimizing environmental impact.

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Enhancing Antibiotic Delivery Using Protein Nanoparticles Against Agrobacterium Tumefaciens

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  1. Retrieved from: http://www.eng.uci.edu/files/images/gallery/Protein_Nanoparticle_Structure.jpg Enhancing Antibacterial Efficacy using Protein Nanoparticles Leslie Tan Zheng Yu Tan Jing Chong Erik Warnquist VarunKulkarni

  2. Introduction • Pesticides are used to eradicate Agrobacterium tumefaciens • High percentage of pesticide does not reach the target species. • Result in water and soil pollution. • Threatens biodiversity.

  3. Introduction • Usage of nanoparticles as drug carrier for pesticides • Increase in therapeutic efficacy • Increasing localisation to diseased sites • Decrease in side effect • Protein Nanoparticle are biodegradable, metabolisable and non-antigenic • Does not accumulate in tissue

  4. Objective • To compare the effectiveness of antibiotic loaded albumin nanodroplets against antibiotic loaded albumin nanofibre on A. tumefaciens, grown both in vitro and in vivo.

  5. Hypothesis • The two delivery techniques will be comparable, through both qualitative and quantitative means

  6. Variables

  7. Equipment • Electrospinning apparatus • Scanning electron microscope (SEM) • Homogenizer • Incubator • Environmental chamber • Spectrophotometer

  8. Materials • Bovine Serum Albumin • Alcohol • A. tumefacians • Potato strips • Diffusion assays • tetracycline and ampicillin

  9. Preparation of albumin nanodroplets

  10. Preparation of albumin nanofibers

  11. Antibiotic loading - nanodroplets • Incubating nanoparticles in antibiotic solution • Antibiotic contained in nanoparticles • Done at protein's isoelectric point • Minimum solubility and maximum absorption • BSA: pH of 4.4 • Larger amount of antibiotic loaded • Antibiotic entrapment efficacy measured

  12. Antibiotic loading - nanofibres • Antibiotics mixed in albumin solution • Homogenous solution • Hypothesis that spinning solution will result in the non polymer antibiotics also being spun

  13. Effectiveness of antibiotic-loaded nanoparticles • Protein nanoparticles digested by proteases to release antibiotics • Antibiotic-loaded nanoparticles are subjected to: • A.tumefacians agar plates discs • A.tumefacians-potato strips

  14. Timeline (HCI)

  15. Timeline (AOS)

  16. References • Buschle-Diller, G., Cooper, J., Xie, Z., Wu, Y., Waldrup, J., & Ren, X. (2007). Release of antibiotics from electrospunbicomponent fibers. Cellulose, 14(6), 553- 562 • Collins, A. (2001). Agrobacterium tumefaciens. Department of Plant Pathology, University of North Carolina State. Retrieved September 19, 2010 from: http:/www.cals.ncsu.edu/course/pp728/ Agrobacterium/Alyssa_Collins_profile.htm

  17. Frenot, A., & Chronakis, I.S. (2003). Polymer nanofibers assembled by electrospinning. Current Opinion in Colloid and Interface Science, 8(1), 64-75. • Hyuk, Y.S., Taek, G.K., & Park, T.G. (2009). Surface-functionalized electrospunnanofibers for tissue engineering and drug delivery. Advanced Drug Delivery Reviews, 61(12), 1033-1042. • Jahanshahi, M. & Babaei, Z. (2008). Protein nanoparticle: A unique system as drug delivery vehicles. African Journal of Biotechnology, 7(25), 4926-4934.

  18. Knee, M., & Nameth, S. (2007). Horticulture and Crop Science: Bacteria. The Ohio State University, Horticulture Department. Retrieved September 12, 2010 from : http://www.hcs.ohio- state.edu/hcs300/bact.htm • Kratz, F. (2008). Albumin as a drug carrier: Design of prodrugs, drug conjugates and nanoparticles. Journal of Controlled Release, 132(3), 171-183. • McManus, P. (2007). Antibiotic Use in Plant Disease Control. Fruit Pathology: University of Wisconsin-Madison. Retrieved September 13, 2010 from: http://www.plantpath.wisc.edu/fpath /antibiotic-use.htm

  19. M.R., Jahanshahi, M., & Najafpour, G.D. (2006). Production of biological nanoparticles from bovine serum albumin for drug delivery. African Journal of Biotechnology, 5(20), 1918- 1923.

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