1 / 20

SuperArray Bioscience Corporation June 2003 Updates

SuperArray Bioscience Corporation June 2003 Updates. New Product Lines: MultiGene-12™ RT-PCR Profiling Kits SureSilencing™ siRNA Kits. PCR & RT-PCR Related Products: MultiGene-12™ RT-PCR Expression Profiling Kits. New Array Verification and Gene Expression Profiling Methods from SuperArray.

inez
Télécharger la présentation

SuperArray Bioscience Corporation June 2003 Updates

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. SuperArray Bioscience Corporation June 2003 Updates New Product Lines: MultiGene-12™ RT-PCR Profiling Kits SureSilencing™ siRNA Kits

  2. PCR & RT-PCR Related Products:MultiGene-12™ RT-PCR Expression Profiling Kits New Array Verification and Gene Expression Profiling Methods from SuperArray

  3. Advantages of MultiGene-12™RT-PCR Profiling Kits • Pathway-specific sets of genes • Profiles eleven closely related genes in a single analysis • Processes up to eight experiments simultaneously • Ideal for multi-gene profiling and array verification • Contains carefully designed and tested primers • Provides a simple way to multiplex RT-PCR analyses

  4. MultiGene-12™ Kit Contents • Kit Contents: • PCR-1000A: ReactionReady™ PCR master mix 1000 • Lyophilized Powder for 96 Reactions (8 tubes, 12 reactions per tube) • Taq DNA Polymerase, Buffer, Nucleotides (dNTPs) • Gel loading dye for end point analysis by agarose gel electrophoresis • MultiGene-12™ Primer Strips: • Eight identical 12-well strips. Each tube represents one gene. • Eleven closely-related genes from one biological pathway • Twelfth tube contains GAPD for normalization

  5. Additional Materials Required Accessory Products (Sold Separately): C-01: ReactionReady™ First Strand cDNA Synthesis Kit* Enough reagents for 8 reverse transcription reactions Buffers RI, RE, P, BC, H2O * At this time, not available in all international territories. Or Individual Components from Promega: Reagent Product Random Primers Promega Catalog # C118 RNase Inhibitor Promega Catalog # N2511 Reverse Transcriptase Promega Catalog # M1701 dNTPs Promega Catalog # U1330

  6. Forward and Reverse Primer Sets for Individual Genes ReactionReady™ PCR master mix 1000 PCR-1000A Experimental RNA 1 2 3 4 5 6 7 8 aliquot Experiments PH-nnn or PM-nnn C-01 37 oC, 60 min ReactionReady™ First Strand cDNA Synthesis Kit* * At this time, not available in all international territories. PCR Overview of MultiGene-12™ RT-PCR Profiling Kit Procedure Gel Analysis

  7. Initial MultiGene-12™ Product Offering • MultiGene-12™ Human Caspase RT-PCR Profiling Kit PH-001A CASP 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 13 • MultiGene-12™ Human BCL2 RT-PCR Profiling Kit PH-002A BAD, BAK1, BAX, BCL2, BCL10, BCL2A1, BCL2L2, BCLxL, BCLxS, BNIP3, BIK • MultiGene-12™ Human Integrin-I RT-PCR Profiling Kit PH-003A ITGA 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 2B • MultiGene-12™ Human Integrin-II RT-PCR Profiling Kit PH-004A ITGA 11, ITGAL, ITGAM, ITGAV, ITGAX, ITGB1, 2, 3, 4, 5, 6

  8. Initial MultiGene-12™ Product Offering • MultiGene-12™ Human MMP-I RT-PCR Profiling Kit PH-005A MMP 1, 2, 3, 7, 8, 9, 10, 11, 12, 13, 14 • MultiGene-12™ Human MMP-II RT-PCR Profiling Kit PH-006A MMP 15, 16, 17, 20, 24, 26, MMPL1, TIMP1, 2, 3, 4 • MultiGene-12™ Human Cytokine-I RT-PCR Profiling Kit PH-007A IL1B, 2, 4, 5, 6, 8, 11, 12A, 13, 15 • MultiGene-12™ Human Cytokine-II RT-PCR Profiling Kit PH-008A IL16, 17, 18, 25, IFNG, IL1A, CSF2, MIF, TGFB1, 3, LTA

  9. MultiGene-12™ Products Coming Soon • Equivalent Products for Mouse • PM-001 through PM-008 • MultiGene-12™ Mouse RT-PCR Profiling Kit • Eight new products every month • Human and Mouse products released on alternate months

  10. TGFb1 TGFb3 GAPD CSF2 IL17 LTA IL1A IL16 IFNg MIF IL18 IL25 RNA from Jurkat cells: Stimulated With PMA and A23187 Unstimulated Differential Gene Expression Observed with a MultiGene-12™ RT-PCR Profiling Kit MultiGene-12™ Human Cytokine II RT-PCR Profiling Kit

  11. RNA Interference Products:SureSilencing™ siRNA Kits Knock down the expression of key genes in specific biological pathways

  12. The siRNA Concept Hutvagner and Zamore (2002) Curr. Opin. Genet. Dev.12: 225-232.

  13. T7 Promoter How we generate siRNA Populations PCR Gene-specific cDNA fragments Same ones used for our GEArrays T7 RNA Polymerase RNase III siRNA

  14. Negative Control siRNA SureSilencing™ siRNA LipofectAMINE2000™ (Invitrogen) transient transfection CELLS CELLS 24 to 48 h incubation Overview of SureSilencing™ siRNA Kit Procedure Harvest cells for RNA. Perform RT-PCR Verification. Verify decrease in message level of specific gene relative to negative control sample. Perform other biochemical, molecular or cell biological assay.

  15. Advantages of SureSilencing™ siRNA Kits • Knock down key genes in specific pathways • Pre-tested & validated. Over 70% suppression. • Ready to use for transfection • No enzymatic steps required • Includes negative control siRNA • Includes primers to verify down-regulated by RT-PCR

  16. SureSilencing™ siRNA Kit Contents • Kit Contents: • Gene-Specific siRNA population • To known down expression of gene by at least 70% • Enough for 90 transfections (24-well format) • Negative Control siRNA population • To control for non-specific effects • To normalize other assay results • Enough for 18 transfections (24-well format) • Gene-Specific Primer Pair • To verify decrease in gene expression • Enough for 100 polymerase chain reactions

  17. Additional Materials Required Accessory Products (Sold Separately): C-01: ReactionReady™ First Strand cDNA Synthesis Kit* Enough reagents for 8 reverse transcription reactions Buffers RI, RE, P, BC, H2O P-1000A: ReactionReady™ PCR master mix 1000 Enough lyophilized master mix for 96 reactions Eight tubes, twelve reactions each * At this time, not available in all international territories. Or Individual Components from Promega: Reagent Product Random Primers Promega Catalog # C118 RNase Inhibitor Promega Catalog # N2511 Reverse Transcriptase Promega Catalog # M1701 dNTPs Promega Catalog # U1330 Taq DNA Polymerase Promega Catalog # M2661

  18. Initial SureSilencing™ Product Offering • RH-0001 APC Adenomatosis polyposis coli • RH-0002 YY1 YY1 transcription factor • RH-0003 E2F1 E2F transcription factor 1 • RH-0004 BRCA1 Breast cancer 1, early onset • RH-0005 BAD BCL2-antagonist of cell death • RH-0006 MYC c-myc proto-oncogene • RH-0007 STAT1 Signal transducer and activator of transcription 1 • RH-0008 FADD Fas-associated via death domain • RH-0009 BCL2 B-cell CLL/lymphoma 2 • RH-0010 LCK Lymphocyte-Specific Protein Tyrosine Kinase • Product base could potential be extended to most of our gene list. • Priority assigned to key upstream regulators of biological pathways.

  19. SureSilencing™ Products Coming Soon • Product base could potential be extended to most of our gene list. • Priority assigned to key upstream regulators of biological pathways. • NFkB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 • CDKN2A Cyclin-dependent kinase inhibitor 2A • EGFR Epidermal growth factor receptor • MAPK1 Mitogen-activated protein kinase 1

  20. GEArray™ cDNA Arrays Kits MultiGene-12™ RT-PCR Profiling Kits SureSilencing™ siRNA Kits Relationship Between SuperArray Product Lines

More Related