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Determination of Pharmaceuticals in Local Water Supplies

Determination of Pharmaceuticals in Local Water Supplies. Maximillian Zart Dr. Joe Gorse. Phases of our Determination. Sample Collection Sample Preparation Sample Testing Analysis. Sample Collection. Sources are all around us Local sources are of key interest in this determination.

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Determination of Pharmaceuticals in Local Water Supplies

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  1. Determination of Pharmaceuticals in Local Water Supplies Maximillian Zart Dr. Joe Gorse

  2. Phases of our Determination • Sample Collection • Sample Preparation • Sample Testing • Analysis

  3. Sample Collection • Sources are all around us • Local sources are of key interest in this determination

  4. Sample Collection • Containment is important • Without proper containers, reactions can occur prior to analysis • Fisherbrand* Certified Clean Amber Wide-Mouth Packers

  5. Sample Preparation • Sample preparation will be conducted using a method known as Solid Phase Extraction (SPE) • SPE is often used to prepare liquid samples and extract semivolatile to nonvolatile analytes • SPE is a great means tool for sample extraction, pre-concentration, and cleanup

  6. Sample Preparation • For our purposes, solid phase extraction will be used as a pre-concentration technique. • Benefits of SPE include ease of use and rapid sample recovery

  7. Solid Phase Extraction : 5 Step Process

  8. Solid Phase Extraction: 5 Step Process

  9. Solid Phase Extraction: 5 Step Process

  10. Sample Testing • Sample testing will occur primarily using two different methods: • High Performance Liquid Chromatography (HPLC) • Gas Chromatography-Mass Spectrometry (GC-MS)

  11. HPLC

  12. HPLC produces a chromatographic representation of various molecules detected in a sample. Retention Times and k’ values are used to determine chemical make-up HPLC

  13. GC-MS • GC-MS is a combination tool • This tool provides two useful charts needed to determine the chemical make-up of a sample • Determines chemicals based upon a vast library available

  14. Analysis • Chromatograms will be compared and reviewed to determine if pharmaceuticals are prevalent in water supplies.

  15. Why? • Previous research has been done showing that steroids, estrogen, and other biologically active species are being found in water supplies. • Research has shown that biologically active compounds have negative effects on wildlife.

  16. References • Kelly, C. J. Chrom. A2000309-314, 872. • Chen, C-Y; Wen, T-Y; Wang, G-S; Cheng, H-W; Lin, Y-H; Lien, G-W Science of the Total Environment 2007 352-365, 378. • Sigma-Aldrich Guide to Solid Phase Extraction19981-12, Bulletin 910.

  17. Thank you • Thanks to: • Dr. Gorse • Mr. Bindis • Students • Gretchen • Dr. Mickley

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