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A Novel Plasma Treatment System for Disinfection of Pharmaceutical Products

A Novel Plasma Treatment System for Disinfection of Pharmaceutical Products. Eoin Byrne Dublin Institute of Technology School of Food Science and Environmental Health Supervisors: Dr. Vasilis Valdramidis and Dr. PJ Cullen. Dr. J.P. Mosnier, J. Connolly. Dr. Kevin Keener. Outline.

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A Novel Plasma Treatment System for Disinfection of Pharmaceutical Products

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  1. A Novel Plasma Treatment System for Disinfection of Pharmaceutical Products Eoin Byrne Dublin Institute of Technology School of Food Science and Environmental Health Supervisors: Dr. Vasilis Valdramidis and Dr. PJ Cullen Dr. J.P. Mosnier, J. Connolly Dr. Kevin Keener

  2. Outline • Introduction • Disinfection • Plasma • PK-1 Device • Methodology • Results • Discussion • Conclusion

  3. Disinfection • Removal of microbes from inanimate surface • Used in water processing, food and pharmaceutical industries • Most types of non-sterile products are not disinfected before packaging

  4. Plasma • Ionised Gas • Fourth state of matter • Highly Conductive • Constitutes 99% of the universe although limited occurrence on Earth

  5. Types of Plasma • Thermal (Hot) Plasma • Non-thermal (Cold) Plasma • Can be generated in atmospheric conditions • Generation of non-thermal plasma • Radio Frequency • Microwave Frequency • Dielectric Barrier Discharge • Type of gases • Helium gas • Nitrogen/Oxygen Mixtures

  6. Plasma • Mode of Microbial Inactivation • Production of radical species • Production of ultraviolet light • Physical damage to microbial cell Scanning electron micrograph image of E. coli after plasma treatment (X10,000)

  7. PK-1 Device • PK-1 is a Dielectric Barrier Discharge Device • Previously demonstrated microbial inactivation in food treatment • Application for in-package treatment • Uses two electrodes coated with PVC • Low power consumption

  8. Objectives • Investigate the effect of plasma on microbial resistance • Assess the microbial survival during storage • Investigate the effect on microbial inactivation of helium and air

  9. Methodology 1. 2. 3. Surface inoculation of agar-agar plates and placement in plastic bags Inoculum preparation (18 hours) Centrifugation (X3 10,000 rpm) 4. 5. Plasma treatment 5min  storage @ 1.5, 3, 18, 21 and 24 hours Serial dilutions and plating on TSA agar followed by incubation at 37oC/48 hours

  10. Methodology: Step 3 • Agar-agar plates inoculated with Listeria innocua • Samples placed into plastic resealable bags and filled with helium gas or air

  11. Methodology: Step 4 Diagram of the in-package system during plasma treatment

  12. Methodology: an example Example of plasma streaming during treatment of an apple

  13. Plasma diagnostics Optical emission spectrum of air/helium DBD plasma measured under low resolution conditions ICCD images of air/helium plasma in a dielectric barrier discharge

  14. Results Effect of helium generated plasma Effect of air generated plasma

  15. Results Graph showing log reduction of L. innocua after plasma treatment using air

  16. Results Graph showing log reduction of L. innocua after plasma treatment using helium

  17. Discussion • Difference between the consistency of the plasma treatment using helium or air • Most microbial inactivation occurred within 1.5 hours • A 2 log reduction in the population of L. innocua using the PK-1 device is possible

  18. Conclusion • The PK-1 device is capable of microbial inactivation after packaging • Helium produces a more consistent inactivation curve during plasma treatment than air • The PK-1 system has potential in pharmaceutical industry

  19. A Novel Plasma Treatment System for Disinfection of Pharmaceutical Products Eoin Byrne Dublin Institute of Technology School of Food Science and Environmental Health Supervisors: Dr. Vasilis Valdramidis and Dr. PJ Cullen Dr. J.P. Mosnier, J. Connolly Dr. Kevin Keener

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