1 / 7

aims to establish a catalogue of all organisms ( 10-30 million species)

Bar Coding. aims to establish a catalogue of all organisms ( 10-30 million species) Ultimately a small portable hand held device will be used to identify samples using information held on a central database.

jacob-cervantes
Télécharger la présentation

aims to establish a catalogue of all organisms ( 10-30 million species)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Bar Coding • aims to establish a catalogue of all organisms (10-30 million species) • Ultimately a small portable hand held device will be used to identify samples using information held on a central database. • The project has begun by barcoding fish, plants, birds, and Lepidoptera (butterflies and moths).

  2. Bar Code Applications Identifying previously undescribed or cryptic species. Food chain analysis of gut contents or animal droppings. Border protection and control: • Identification of agricultural pests and invasive species. • Identification of protected species. Enforcement of food manufacturing and labeling laws: • Analysis of food product for prohibited ingredients. • Verification of product authenticity. Analysis of animal feed for prohibited components (e.g animal tissue which may cause BSE). Hazard analysis (e.g. which bird species is the major contributor to bird strike at airports?).

  3. Mitochondrial DNA • mitochondrial gene found in all plants and animals. • base sequence is genetically stable because it is inherited from the mother only. It contains no inserted or deleted DNA. This allows for easy genetic comparison.

  4. The CO1 gene • Cytochrome c oxidase subunit 1 • Slight different between species. • Allows for unique identification of species. • Intra-species variations generally small (<2%). • CO1 easily recovered from samples. • Fast and inexpensive analysis.

  5. CO1 gene cannot be used for: • Plants, bacterium and some animals. • Groups with high intraspeciesvariability for the CO1 gene (e.g. amphibians). • Groups with <2% sequence diversity for CO1 between species (variability too low for accurate differentiation • plants • Recently diverged species • Hybrid species where the offspring is a result of the mating between a male and female from different species. • Organisms containing nuclear pseudogenes (inactive copies of genes with multiple mutations and/or deletions).

  6. Bar Coding Data analysis: Specimens identified by finding the closest matching reference record in the database. Laboratory analysis: DNA extraction Amplification (PCR) Clean up Sequencing Database inclusion: Barcode of life database (BOLD) International nucleotide sequence database collaborative Specimen collection

More Related