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Advanced Enzyme-Based Analysis for Neurointensive Care Monitoring

Explore highly sensitive enzyme-based analysis for real-time monitoring of glucose and lactate in neurointensive care. Efficient conversion in a packed bed of enzymes allows for precise detection. Data recorded every 3 minutes or every 30 seconds for optimal insights.

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Advanced Enzyme-Based Analysis for Neurointensive Care Monitoring

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  1. Controlling the sample

  2. features • Species diffuse through dialysis tube into clean buffer • Flow injection analysis: clean background • Highly sensitive enzyme-based analysis

  3. linear response to hydrogen peroxide is observed in the concentration range 5 x 10-8 – 6 x 10-6M.

  4. Low concentrations of ferrocene in the buffer Primary reaction on oxidase enzyme is therefore with oxygen Efficient conversion in a packed bed of enzymes Flow injection analysis gives high sensitivity on a clean background

  5. Fig. 5. (A) A 2 h period of on-line monitoring of glucose (black) and lactate (grey) present in dialysate, from a probe implanted in the brain of a patient in the neurointensive care unit. Data recorded every 3 min. (B) A 2 h period of on-line monitoring of glucose (black) and lactate (grey) present in dialysate, from a probe implanted in the brain of a patient during surgery to clip an aneurysm. Data recorded every 30 s.

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