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+thiamine

A. G2/M arrest 36°C 3.5 hr. +thiamine. +auxin. 25°C 25°C release. (asyn) (-3.5h) (time 0). B. time after G2/M release. time after G2/M release. asyn -3.5h 0 30 60 90 (min).

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+thiamine

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  1. A G2/M arrest 36°C 3.5 hr +thiamine +auxin 25°C 25°C release (asyn) (-3.5h) (time 0) B time after G2/M release time after G2/M release asyn -3.5h 0 30 60 90 (min) asyn -3.5h 0 30 60 90 (min) Cdc45 tubulin Pol1 tubulin * 1 2 3 4 5 6 1 2 3 4 5 6 C cdc45-off-aid pol1-off-aid no depl (+HU) Pol1 depl no depl (+HU) Cdc45 depl (min) 180 120 90 60 0 (min) 180 120 90 60 0 1C 2C 1C 2C 1C 2C 1C 2C

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