1. Association of melanocortin 4 receptor (MC4R) polymorphism with body composition and carcass traits of commercial pigs in Thailand body composition carcass traits EBV genotype Selection Required less time to apply

2. Association of melanocortin 4 receptor (MC4R) polymorphism with body composition and carcass traits of commercial pigs in Thailand Traits for study BL, body length (cm) SL, shoulder length (cm) HL, ham length (cm) RL, rump length %Lean, percent lean aLAM, loin eye area muscle adjust at 104 kg (kg) D104, day during birth to body weight at 104 kg (day) aBF, backfat thickness adjust at 104 kg (cm) back

3. Association of melanocortin 4 receptor (MC4R) polymorphism with body composition and carcass traits of commercial pigs in Thailand digested by the restriction endonucleaseTaq I AA AA AG AG GG 483 bp 500 bp 407 bp 76 bp Figure 2 RFLP fingerprint on 2% agarose. AA = 483 bp AG = 483,407 and 76, GG = 407,76

4. Table1. Heritability of the traits (diagonal), genetic correlation (above diagonal) and phenotypic correlation (belowdiagonal) rg Low to Moderate Genetic response slow 1 BL, body length (cm); SL, shoulder length (cm); RL, rump length; HL, ham length (cm); aBF, backfat thickness adjust at 104 kg (cm); aLAM, loin eye area muscle adjust at 104 kg (kg); %Lean, percent lean; D104, day during birth to body weight at 104 kg (day).

5. Association of melanocortin 4 receptor (MC4R) polymorphism with body composition and carcass traits of commercial pigs in Thailand Conclusions Slow h2 Low 1) Body composition and D104 year 2) Body composition rg Low to Moderate 3) Polymorphism of MC4R may not be appropriate to use as candidate gene in this populations - to confirm the result, many sample must be included

6. Introduction • Good meat texture • Low cholesterol • Good taste • Price higher 2-3 times Thai Native Chickens Broiler ? ? ? Low growth rate Improve growth traits Growth traits Candidate genes approach (Wattanachant et al., 2004; Jaturasitha et al., 2008)

7. Materials and methods (60 chickens/breed) Pradoo Hang Dam Chee Data Body Weight (0, 4, 8, 12, 16 Wks) Average Daily Gain (ADG) (0- 4, 0-8, 0-12, 0-16 Wks)

8. Results and Discussion Marker- traits Analysis IGFBP2 IGF-I cGH GHSR Not Associated T>C A>C G>A C>T 5’ UTR Intron1 Intron3 Intron2 Not Associated EBV data Pradoo Hang Dam Chee

9. Conclusions Not Associated Chee Pradoo Hang Dam IGF-I cGH marker-assisted selection Improve growth traits