1 / 10

ANTIBIOTICS & ANTISEPTICS

ANTIBIOTICS & ANTISEPTICS. Lab 11. ANTIMICROBIALS. An antimicrobial is a substance that kills or inhibits the growth of microorganisms ACTION OF ANTIMICROBIAL DRUGS Bacteriocidal (destroy microbes) Bacteriostatic (Inhibit growth) LABORATORY TESTS IN CHEMOTHERAPY Susceptibility Testing

jela
Télécharger la présentation

ANTIBIOTICS & ANTISEPTICS

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. ANTIBIOTICS & ANTISEPTICS Lab 11

  2. ANTIMICROBIALS • An antimicrobial is a substance that kills or inhibits the growth of microorganisms • ACTION OF ANTIMICROBIAL DRUGS • Bacteriocidal (destroy microbes) • Bacteriostatic (Inhibit growth) • LABORATORY TESTS IN CHEMOTHERAPY • Susceptibility Testing • Disk Diffusion Methods • Minimal Inhibitory Concentration (E test estimates) • Broth Dilution Methods • MIC, & Minimal Bacteriocidal Concentration (MBC)

  3. Antibiotics • An antibiotic is a substance produced by a microorganism that is antagonistic to the growth of other microorganisms • SPECTRUM OF ANTIMICROBIAL ACTIVITY • Broad spectrum antibiotics • Narrow spectrum antibiotics Testing the susceptibility of Staphylococcus aureus to antibiotics by the Kirby-Bauer disk diffusion method. Antibiotics diffuse out from antibiotic-containing disks and inhibit growth of S. aureus resulting in a zone of inhibition

  4. DISK DIFFUSIONMETHODS BHA Medium

  5. DISK DIFFUSION METHODS

  6. CHEMICAL METHODS OF CONTROL • DISINFECTANTS • Bacteriocidal (destroy microbes) • Bacteriostatic (Inhibit growth) • ANTISEPTICS • Ideal Disinfectant/Antiseptic • Concentration of the active agent • Environmental factors • Temperature, nature of suspending medium, presence of organic matter • The particular microbe • The number of microbes • Exposure time

  7. EVALUATING DISINFECTANTS • DISK DIFFUSION METHODS STREAK PLATE TO OBTAIN A CONFLUENT BACTERIAL LAWN

  8. EVALUATING DISINFECTANTS • DISK-DIFFUSION METHODS

  9. EVALUATING DISC DIFFUSION METHODS FOR VARIOUS DISINFECTANTS USING STANDARD BACTERIA

More Related