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This study outlines the detection of various marine Synechococcus strains using specific probes targeting E. coli sequences. Probes designed for strains such as WH8103, CC9311, WH7805, and others were evaluated for their effectiveness in identifying target sequences. The investigation focuses on the position of sequences in the 5'-3' direction, emphasizing the diverse genetic makeup of different Synechococcus clusters and their exclusion of certain Prochlorococcus strains. The findings contribute to understanding marine cyanobacterial diversity and effective detection methods.
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Synechococcus Probes Probe E.coli Target Sequence Strains detected position 5’-3’ 1 184 CCGCGAGGTGAAATGAAT All WH8103 cluster 2 588 CCCWACAAGTCTGCTGTT CC9311 & Almo3 3 1008 CTTGGAAACGAGAGGGTG WH7805 / WH7803 cluster 4 1018 GAGAGAGTGCCCTCGGGA Eum14 & Oli31 5 1007 TCTGGGAAACCGGAGGGT Minos 1 & Minos 11 6 1281 TCTCATAAAAAGTGGCTC All WH7805 / WH7803 / Oli31 cluster 7 1258 GCAAGTTCGCGAGGACAA All marine Synechococcus except WH8101, no Prochlorococcus except MIT9303 8 620 TAACTCCATCATGGCAGT All marine Synechococcus, except Minos 1 & Minos 11, & all Prochlorococcus 9 1038 CAGTGACAGGTGGTGCAT All marine Synechococcus & Prochlorococcus, ACE / PS680 cluster, PCC9005