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Erythrocyte Sedimentation Rate (ESR) Procedure: Equipment, Specimen Handling, Reading & Reporting Results

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Learn about the ESR test procedure, from specimen preparation to reading results. Includes factors affecting ESR levels, equipment needed, reference values, and quality assurance tips.

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Erythrocyte Sedimentation Rate (ESR) Procedure: Equipment, Specimen Handling, Reading & Reporting Results

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  1. به نام هستي بخش يكتا

  2. Definition ErythrocyteSedimentation Rate

  3. Principle

  4. Affected Factor • Plasma • Fibrinogen- Globulins-Albumin • RBC • Macrocyte-Sickle-Aniso-spherocyte

  5. Pipette Colorless 30cm 0-200mm <2.55 mm <5% Clean & dry Pipette Rack +2° No leakage Equipment

  6. Storage Specimen preparation Handling of pipette Reading result Reporting result

  7. Procedure - selected • Blood Collection • Vein less than 30 min • K2EDTA 1.4-2mg/ml K3EDTA 1.6-2.4 Na2EDTA 1.4-2 • Citrate 3.2% 4 volume blood+1 volume Normal salin 8.5 g/l

  8. ESR , 1 hour = x mm • Stability 4 hours at room temperature(18-25 C) 24 hours (4 C) must be warmed before test • 12 times inversion • Pipette ( no mouth) • 60+ 1 minute

  9. Reference value • > 50 y • Men 20 • Women 30 Men=age/2 • < 50 y • Men 15 women=(age+10)/2 • Women 20 • > 80 y • Men 30 • Women 42

  10. Reference method • Better CV • Adjust PCV<0.35 • Sample is divided 23.5 ml centrifuge Plasma remove add to

  11. Volume of plasma ESR ref 1 hour = x mm At last check PCV

  12. Corrected ESR= ( undiluted  0.85) – 12 Table <95%

  13. Quality Assurance • Equipment • Procedure • No control ( except for automation) we can not calibrate but it is stable

  14. control • Patient sample Reference method routine • Compare them with table Reference = 33 Routine 11-26

  15. Disposal • Wash with tap water acetone dry

  16. Source of Error

  17. citrate 3.2% / 3.8% Osmolarity HCT Kits

  18. Preparation • 32 gr Na3C6H5O7.2H2o / 1L DW • 121º 15min • 4 months at 4 º • 1/4 for ESR • 1/9 for Coagulant assay

  19. Correction • 1- Formula • ( 100-PCV)/(595-PCV)= cc citrate / 1 cc Example:HCT=55  (100-55)/(595-55)=0.08 1-0.08=0.92 0.92 blood + 0.08 citrate

  20. curve

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