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Simultaneous Quantification of Lornoxicam and Paracetamol by First Derivative UV-Spectroscopy

A precise method utilizing derivative spectroscopy to quantify Lornoxicam and Paracetamol in combined tablet dosage forms, offering high sensitivity and wide linearity range along with rapid and eco-friendly analysis.

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Simultaneous Quantification of Lornoxicam and Paracetamol by First Derivative UV-Spectroscopy

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  1. Simultaneous Quantification of Lornoxicam and Paracetamol Combination By Application of First Derivative UV- Spectroscopy SwethaBhavani N, HimaBindu S, Venkateswara Rao A, Panikumar D Anumolu

  2. 1 Conclusion Materials & methods

  3. DERIVATIVE SPECTROSCOPY 2 Derivatization of actual spectra with respect to wavelength Zero order First order Second order Zero, first and Second-order UV derivative spectrum

  4. 3 Advantages of First derivative spectroscopy: (1) Precise determination of the λmaxcan be obtained from the zero crossing of the first derivative. (2) Improved spectral resolution (3) Discrimination of broad bands Resolution enhancement in derivative spectroscopy

  5. OBJECTIVES 4

  6. Drug profile of Lornoxicam 5 Category: NSAID Chemical name: 6-Chloro-4-hydroxy-2-methyl-N-2pyridinyl-2H-thieno[2,3-e]-1,2thiazine-3-carboxamide1,1-dioxideMolecular formula:C13H10Cl N3O4S2 Molecular weight: 371.82 g/mol Melting point: 225-2300c Solubility: Slightly soluble in chloroform & 0.1mol/LNaOH, and very slightly soluble in methanol and acetonitrile, hardly soluble in water. pKa: 4.7Partition coefficient : Log P (octanol/pH 7.4 buffer):1.8 ,octanol-water-3.15(calc)

  7. Drug profile of Paracetamol: 6 Category:Analgesic and antipyretic Chemical name: N-(4-Hydroxyphenyl)acetamide Molecular formula:C8H9NO2 Molecular weight:151.2 Melting point: 169.0° to 170.5° Solubility: Very slightly soluble in cold water, considerably more soluble in hot water; soluble in ethanol, methanol, dimethylformamide, ethylene dichloride, acetone, and ethyl acetate; very slightly soluble in chloroform; slightly soluble in ether; practically insoluble in petroleum ether, pentane, and benzene pKa: 9.5 (25°).Partition coefficient : Log P(octanol/water), 0.5. Appearance: White crystals or crystalline powder

  8. Materials and methods 7 Instrument : Shimadzu UV-1800 Spectrophotometer

  9. 8 Preparation of standard stock solutions S.No.

  10. 9 A B S O R B A N C E WAVE LENGTH(nm)

  11. 10 גmax for LOR zcp for LOR dA/dג zcp for PAR גmax for PAR WAVELENGTH

  12. 11

  13. 12 zcp for LOR גmax for LOR dA/dג zcp for PAR ג max for PAR WAVELENGTH (nm)

  14. 13 Concentration(mcg/ml) Derivative absorbance

  15. 14 Derivative absorbance Concentration(mcg/ml)

  16. Precision 15

  17. 16

  18. 17 System Suitability Parameters

  19. Assay 18

  20. 19 ADVANTAGES OF PROPOSED METHOD Simple spectra and less interference. High sensitivity and wide linearity range. Rapid, economical. Eco-friendly, accurate and precise method. High spectral discrimination. Single analytical method for quantification of LOR and PAR in combined tablet dosage forms.

  21. 20 It is a simple, novel, more economic, reliable, precise and accurate method for simultaneous estimation of Lornoxicam and Paracetamol in bulk and formulations. So this method can be successfully applied to routine analysis of studied drugs in their tablet dosage forms.

  22. References: 21 • ICH Harmonized Tripartite guideline validation of analytical procedures: text and methodology, Q2(R1) Current step 4 version, 27 october 1994. • Mark, H. and Workman, J. Derivatives in spectroscopy. Spectroscopy. 2003 ,18 (4): 32-37 • Beckett AH and Stenlake JB. Practical pharmaceutical chemistry; 4th edition, the athlone press. 2007, 269-299.

  23. 22 • Lakshmi Sivasubramanian*, Lakshmi K.S. and TinTu.T. PB Khedikar and G Pawnikar, Absorption Correction Method for Simultaneous Estimation of lornoxicam and paracetamol tablet dosage form by absorbance ratio method , International Journal of pharmacy and pharmaceutical sciences, 2010,vol 2,Issue 4 . • DharaJ.Patel*Vivek Patel., Simultaneous determination of paracetamol and lornoxicam in tablets by thin layer chromatography combined with densitometry, An International Journal of chem tech research, pp 1929-1932,Vol-2 no.4. • T.Raja*,A.LakshmanaRao,Validated High performance Thin layer liquid chromatography method for simultaneous quantitation of paracetamol and lornoxicam in bulk drug and pharmaceutical formulation , International Journal Pharm Biomed Res 2012,3(3),162-166 .

  24. 23 Thanq…

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