SDS protein gel electrophoresis

Feb 22, 2013

70 likes | 684 Vues

Protein Electro Elution Method with GeBAflex-tube. SDS protein gel electrophoresis. Desired protein. Gel slice containing the desired protein. Gel slice containing the desired protein Inserted to GeBAflex-tube . GeBAflex-tube inserted to electro elution supporting tray .

Share Presentation

Embed Code

Link

electro elution
protein electro elution method
electrophoresis apparatus
horizontal
gel slice
gel electrophoresis

jordana

Télécharger la présentation

SDS protein gel electrophoresis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

Protein Electro Elution Method with GeBAflex-tube SDS protein gel electrophoresis Desired protein Gel slice containing the desired protein Gel slice containing the desired protein Inserted to GeBAflex-tube GeBAflex-tube inserted to electro elution supporting tray Electro elution of the protein in an Horizontal electrophoresis apparatus

2D-SDS Gel Electrophoresis

2D-SDS Gel Electrophoresis. July 1, 2003 Theoretical basis for 2D-SDS Gel Elect The SWISS-2DPAGE Database Workshop. 2D-SDS Gel Electrophoresis What is it?.

936 views • 24 slides

Gel Electrophoresis

Contents. Basic PrinciplesThe Electric FieldThe Electrophoretic Mobility ValueThe GelsImproving ResultsMethods of SeparationManipulating the GelsNew Techniques. Principles of Electrophoresis. Separation based upon several factorsMolecular chargeMolecular massMolecular shapeMolecules affec

463 views • 14 slides

GEL ELECTROPHORESIS

GEL ELECTROPHORESIS. Gel electrophoresis is a process used by scientists to separate mixtures of molecules by size, creating a DNA “fingerprint” It is most often used to separate molecules of proteins and molecules of DNA. Everyone has their own unique DNA (unless you have an identical twin)

1.04k views • 19 slides

SDS-Polyacrylamide Gel Electrophoresis of Proteins

SDS-Polyacrylamide Gel Electrophoresis of Proteins. a.k.a. SDS-PAGE. NBD-chloride will bind to many of the same places ATP does… however it will also react with the protein and remain attached, unlike ATP.

288 views • 5 slides

Gel Electrophoresis

Gel Electrophoresis. The questions. Why? To separate pieces of DNA by size. How? Use electrical charge to pull the negatively charged DNA through a gel that has small pores in it.

572 views • 32 slides

Protein Gel Electrophoresis

Protein Gel Electrophoresis. Native PAGE Native Gradient PAGE Urea PAGE SDS PAGE SDS Gradient PAGE IEF 2D PAGE Western Blot. Principle. Proteins move in the electric field. Their relative speed depends on the charge, size, and shape of the protein. From large to small and simple.

1.47k views • 36 slides

SDS PAGE = SDS polyacrylamide gel electrophoresis

s odium d odecyl s ulfate, SDS (or SLS): CH 3 -(CH2) 11 - SO 4 -- CH 3 -CH 2 -CH 2 -CH 2 -CH 2 -CH 2 -CH 2 -CH 2 -CH 2 -CH 2 -CH 2 -CH 2 -SO 4 --. SDS PAGE = SDS polyacrylamide gel electrophoresis. SDS. All the polypeptides are denatured and behave as random coils

1.6k views • 79 slides

SDS- Polyacrylamide Gel Electrophoresis

Experiment 7 . SDS- Polyacrylamide Gel Electrophoresis. BCH 333[practical] . Objectives : To be familiar with SDS-PAGE protocols (Linear slab gel ). SDS- Polyacrylamide Gel Electrophoresis.

889 views • 21 slides

SDS-Polyacrylamide Gel Electrophoresis

Lab#4. SDS-Polyacrylamide Gel Electrophoresis. BCH 462 [practical ] . Objectives: -Separation of protein fractions using SDS-PAGE. SDS- Polyacrylamide Gel Electrophoresis.

443 views • 15 slides

Gel Electrophoresis

Gel Electrophoresis. Lab.8. Gel Electrophoresis. Gel Electrophoresis. Gel electrophoresis apparatus. An agarose gel is placed in this buffer-filled box and electrical field is applied via the power supply to the rear .

835 views • 14 slides

Gel Electrophoresis

Gel Electrophoresis. First, What……. Simply put, gel electrophoresis is a technique used to separate molecules such a DNA, RNA and proteins according to size. Requires an electric current as these molecules are negatively charged

478 views • 10 slides

Laboratory : Experiment 2: SDS PAGE Lecture : SDS Polyacrylamide Gel Electrophoresis

Laboratory : Experiment 2: SDS PAGE Lecture : SDS Polyacrylamide Gel Electrophoresis In-Class Writing : review proposals (pages xxxiii-xxxvi & 129-133) Read : Unit 2 (34-35) & JBC 266: 3811-14 (1991) Hand In : proposal (3 copies) Due Next Class : flow chart 4 (day 1; DNA extract).

447 views • 9 slides

Gel Electrophoresis

Gel Electrophoresis. Resource: http://web.utk.edu/~khughes/GEL/sld001.htm. What is Gel Electrophoresis?. Gel Electrophoresis separates molecules on the basis of their charge and size . The charged macromolecules migrate across a span of gel because they are placed in an electrical field .

479 views • 14 slides

Gel Electrophoresis

Gel Electrophoresis. What is Gel Electropho.

357 views • 14 slides

Gel Electrophoresis

Gel Electrophoresis. Digital image of 3 plasmid restriction digests run on a 1% w/v agarose gel, stained with ethidium bromide. The DNA size marker is a commercial 1 kbp ladder. The position of the wells and direction of DNA migration is noted.

293 views • 5 slides

Gel Electrophoresis

Gel Electrophoresis. A molecular biology tool. Purpose . To separate and analyze/compare fragments of DNA. 4 Main Steps:. 1) Restriction Enzymes: Either one or several R.E.’s is added to a sample of DNA. Enzymes cut them into fragments. 2) Prepare Gel:

458 views • 35 slides

SDS-PAGE Sodium Dodecyl Sulfate - Polyacrylamid Gel Electrophoresis

SDS-PAGE Sodium Dodecyl Sulfate - Polyacrylamid Gel Electrophoresis. Sodium Dodecyl Sulfate. SDS is a common ingredient in detergents Other names for SDS include laurel sulfate and sodium laurel sulfate As a detergent SDS destroys protein secondary, tertiary and quaternary structure

351 views • 13 slides

Protein Gel Electrophoresis

557 views • 23 slides

Gel Electrophoresis

Gel Electrophoresis. By: Brian Barnes, Gary Pope, Eliza Morgan. What is GE?. Pronounced Gel ee-LEK-tro-fo-REE-sis Technique for separating DNA or Protein Molecules into strands according to length. What’s The Point?. Used to separate DNA and identify strands of interest

444 views • 16 slides

GEL ELECTROPHORESIS

BIOCHEMISTRY

273 views • 12 slides

Gel Electrophoresis

Gel Electrophoresis. Student Instructions Edvotek set up. Salk Institute Mobile Lab. Gel Electrophoresis Student Station Set-up. tip container w/tips. waste container. micropipettor. practice sheet in protector. foam epi rack w/ 1 food color tube each: orange, green, purple.

689 views • 8 slides

More Related