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MALDI-TOF:

MALDI-TOF:. http:// www.raust.is/2003/1/13/raust2003-1-13.pdf. MALDI-TOF:. http:// biopedia.org/index.php/MALDI-TOF-MS. A > H. t.d. C (6e - ). l = 0/s. l = 1/p. l = 2/d. l = 3/f. E. : :. : :. : :. : n = 4. n = 3. n = 2. n = 1. E. E. : :. : :. A(n=3). A(n=3) + B.

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MALDI-TOF:

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  1. MALDI-TOF: http://www.raust.is/2003/1/13/raust2003-1-13.pdf

  2. MALDI-TOF: http://biopedia.org/index.php/MALDI-TOF-MS

  3. A > H. t.d. C (6e-) l = 0/s l = 1/p l = 2/d l = 3/f . E : : : : : : : n = 4 n = 3 n = 2 n = 1

  4. E E : : : : A(n=3) A(n=3) + B n = 3 n = 3 A(n=2) A(n=2) + B n = 2 n = 2 A(n=1) A(n=1) + B n = 1 n = 1 A A - B rAB

  5. 2 AB+ + e AB** 1xhn AB E

  6. 2 AB+ + e AB** 1xhn 2xhn AB REMPI = Resonance Enhanced MultiPhoton Ionization E (2 + 1) REMPI

  7. 2 AB+ + e A + B+ + e AB** 1xhn 2xhn AB REMPI = Resonance Enhanced MultiPhoton Ionization E

  8. 2 AB+ + e AB** 1xhn 2xhn 3xhn nxhn AB E REMPI = Resonance Enhanced MultiPhoton Ionization

  9. Excimer Laser Pellin Broca prism SHG Dye- Laser out Time delay 200-1200 mS SHG control In HX laser control In út oscilloscope computer nozzle lense REMPI-TOF TOF Turbo Pump MCP detector Voltage devider HV -2Kv

  10. LASER radiation of C2H2 Ion yield C2H2+ C+ C2+ H+ Magn jóna C2H+ CH+ CH2+ Mw Time of flight

  11. HCCH*: Orka HCCH:

  12. + + C2 C2 H2 Orka HCCH*:

  13. Orka HCCH*:

  14. C CH2 Orka HCCH*:

  15. LASER radiation of C2H2 Ion yield C2H2+ C+ C2+ H+ Magn jóna CH+ C2H+ CH2+ Mw Time of flight

  16. Big Organic molecules Interstellar space C2H2 ??? : Life(?)

  17. Interstellar space C2H2 ??? C CH2 C2 Big Organic molecules : Life(?)

  18. 2 AB+ + e AB** 1xhn 2xhn 3xhn nxhn AB E REMPI = Resonance Enhanced MultiPhoton Ionization Resonance excitation to high energy states of neutral species, followed by photon ionization; For example:

  19. Intensity Mass spectrum RCl = HCl H35Cl+ H+ Two photon resonance excitation= 82842.36 cm-1 35Cl+ H37Cl+ 12C+ 1 12 35 Mw

  20. H+ H35Cl+ 35Cl+ H37Cl+ Two photon excitation / cm-1 Ion yield REMPI- spectrum Mw / rel. cm-1

  21. 35Cl+ H35Cl+ 37Cl+ Mw H37Cl+ 2xhv H35Cl+ H37Cl+ 35Cl+ /cm-1 Mw / rel.

  22. H37Cl+ 2xhv 37Cl+ /cm-1 H35Cl+ 35Cl+ Mw /amu H+

  23. 2xhv/cm-1 H35Cl+ H37Cl+ H+ 35Cl+ 37Cl+ Mw/amu

  24. 2 AB+ + e A + B+ + e AB** 1xhn 2xhn AB REMPI = Resonance Enhanced MultiPhoton Ionization E

  25. 2 A+ + B + e AB+ + e A + B+ + e AB+ + e A + B+ + e AB** AB # # 1xhn 2xhn AB REMPI = Resonance Enhanced MultiPhoton Ionization E

  26. 2 A+ + B + e AB+ + e AB+ + e A + B+ + e A + B+ + e AB** AB # # 1xhn 2xhn AB REMPI = Resonance Enhanced MultiPhoton Ionization E A+ + e B+ + e A + B/B*

  27. MCP detector Basedon http://en.wikipedia.org/wiki/Photofragment-ion_imaging

  28. = MCP (Micro Channel Plates Basedon http://pubs.rsc.org/en/content/articlelanding/2010/cp/c001233e#!divAbstract = Charge-CoupleDevicecamera http://pubs.rsc.org/en/content/articlelanding/2012/cp/c2cp40822h#!divAbstract

  29. = MCP (Micro Channel Plates http://pubs.rsc.org/en/content/articlelanding/2010/cp/c001233e#!divAbstract = Charge-CoupleDevicecamera http://pubs.rsc.org/en/content/articlelanding/2012/cp/c2cp40822h#!divAbstract

  30. Dt I++e + Br* I++ e + Br Energy I + Br* I + Br r(I-Br) Basedonhttp://www.chem.queensu.ca/people/faculty/Stolow/Research/QCVibrational.html

  31. Laserbeams: Polarizedlight Dt Unsymmetricimages!?

  32. https://www.physics.umn.edu/research/workshops/fluorescence/Fluoro4.pdfhttps://www.physics.umn.edu/research/workshops/fluorescence/Fluoro4.pdf

  33. https://www.physics.umn.edu/research/workshops/fluorescence/Fluoro4.pdfhttps://www.physics.umn.edu/research/workshops/fluorescence/Fluoro4.pdf

  34. Reflectron MS Accelleration region Flight Region Magnetic Reflector

  35. Withoutreflectron: Detector tTOF Withreflectron: tTOF Basedon: http://msr.dom.wustl.edu/reflectrons-ion-mirrors/

  36. 2) Velocity selction by jet cooling / 2) Hraðaval framkvæmt með “gasúðara” Nozzle

  37. Molec.beam Experiments byuse of molecularbeams/ Collision John C. Polanyi Nobelprize1986: DudleyHerzbachYuanLee 1975-1990: Molecularbeam Detection Detector -Collisioncomplexesdeterminedfromscatteringpatterns

  38. Light /Ljós ? White light/Hvítt ljós Rauður Gulur Grænn Blár

  39. l /Bylgju- lengd Prism /Gler- strendingur

  40. Monochromatic light / Einlitt ljós (ein bylgjulengd) • Anisotropic / mjög stefnuháð • High photon concentration /Mikið magn jafnorkuríkra ljóseinda • Pulsed or continuus /örstuttir blossar eða samfelld geislun - LASER beam /LASER geisli: 6 Sample /Sýni;sameindir LASER LASER- beam/geisli

  41. Laser activity /Laser virkni:

  42. He/Ne & Dye laser /Lit-LASER

  43. CO2 and Excimer LASERs

  44. Thechemicallaser /Efna-Laserinn F + H2 HF* (v´ > 0) + H http://perg.phys.ksu.edu/vqm/laserweb/ch-6/f6s1t9p4.htm

  45. Thechemicallaser /Efna-Laserinn

  46. Efna-LASER

  47. LUMO HOMO E R(A-B)

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