Nanocoatings of inorganic surfaces b y molecular biomimetic

1. Molecular Biomimetic: Conception of new materials that imitate, or learn from nature at molecular scale ZnO ZnO • Ag2O • Ag2O 100 µm Nanocoatings of inorganic surfaces bymolecular biomimetic C. VREULS, A. GENIN, G. ZOCCHI, G. GARITTE,C. ARCHAMBEAU, C. VAN DE WEERDT, J. MARTIAL BMGG-CERM-ArcelorMittal Research Ličge Director: J. MARTIALJ.Martial@ulg.ac.be Aim of the Biocoat project (PPP, RW): To develop new functionalities with high added valueon inorganic substrates through multifunctional molecular coatings following a multidisciplinary biomimetic approach Strategies for surface coating: Bottom-up hierarchic assembly using engineered proteins Interests of GEPIs : high specificity for- chemical composition - structuration (powder, surface…) - cristallographic form GEPI Genetically Engineerd Polypeptide for Inorganic small peptide that recognizes and specifically binds an inorganic surface Inorganic-binding peptides as tools for surface quality controlGEPI applications Nature Molecular biomimetic COPY NATURE Patterned substrate: 50-nm layer of Ag on pure zinc ZnO-binding peptide with FITC at the N-terminal Recognition => binding protein - inorganic material GEPI: Linker betweeninorganic-organic materials 2 types of galvanized steel 1st step : isolate GEPI to gather incompatible materials Selection of GEPI by Phage Display technology Galva Gi206031 Galva DRX51 Control quality of raw or coated inorganic surface by fluorescence analyses Perspectives Use Gepi for powder sorting, Surface fonctionalization… 1: Librairy presentation; 2: Wash; 3: Elution; 4: Amplification; 5: next round of selection; 6: sequencing after 3-4 rounds of selection Team’s Publications & Patent ApplicationsAll-in-one strategy for the fabrication of antimicrobial biomimetic films on stainless steel. J. Mat. Chem. 2009 19: 4117-4125. Inorganic-binding peptides as tools for surface quality control J. Inorganic Biochem. 2010Plasma deposition of antimicrobial peptides onto stainless steel for the prevention of bacterial biofilms (submitted to J. Mat. Chem) Biomolecules in multilayer film for antimicrobial and easy-cleaning stainless steel surface applications (submitted to Biofouling) Multifunctional Coatings WO 2009/147007 A2Inorganic-binding peptides as tools for surfaces quality control detection PCT/EP2009/004876). AcknowledgmentsThe research was partly supported by the Walloon Region (PPP program BIOCOAT). We thank the BIOCOAT team members for their contribution, in particular Fabrice Farina (ArcelorMittal Research Industry, Liege, Belgium) for providing the various inorganic material coupons, GIGA-R, Systems Biology and Chemical Biology Unit (ULg)