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Fr-08-2 (main) Saponin-1 (1kg) Saponin-2 (1kg) Saponin-2 (1kg) Saponin-3 (1kg) Saponin-3 (1kg) Saponin-3 (1kg) IPB200-08-1 IPBS200-08-2 IPBS-05 Mobile Phase: CHCl3-MeOH-H2O 7.5:2.5:0.35+8:2:0.2 (1:1). 1 2 3 4 5 6 7 8 9 10.

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  1. Fr-08-2 (main) • Saponin-1 (1kg) • Saponin-2 (1kg) • Saponin-2 (1kg) • Saponin-3 (1kg) • Saponin-3 (1kg) • Saponin-3 (1kg) • IPB200-08-1 • IPBS200-08-2 • IPBS-05 • Mobile Phase: • CHCl3-MeOH-H2O 7.5:2.5:0.35+8:2:0.2 (1:1) 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10

  2. IPBS-01 • IPBS-03 (ilexsaponin A1) • IPBS-04 (ilexsaponin B2) • IPBS-05 (new compound) • PSF • IPBS-06 (new compound) • IPBS-07 (cikusetasaponin Iva) • IPBS-08 (Ilessaponin B3) • IPBS-09 (ilexsaponin B1) • Mobile Phase: • CHCl3-MeOH-H2O 7.5:2.5:0.35 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9

  3. IPBS-01 • IPBS-03 (ilexsaponin A1) • IPBS-04 (ilexsaponin B2) • IPBS-05 (new compound) • PSF • IPBS-06 (new compound) • IPBS-07 (cikusetasaponin Iva) • IPBS-08 (Ilessaponin B3) • IPBS-09 (ilexsaponin B1) • Mobile Phase: • CHCl3-MeOH-H2O 8:2:0.2 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9

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