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This lecture focuses on the critical aspects of protoplast, cell, tissue, and organ culture, alongside in-vitro pollen germination and fertilization. Key topics include protoplasm, cell fusion, and methodologies for crop propagation and somatic embryogenesis, which are essential for the rapid multiplication of agriculture. These techniques play a vital role in rural development, especially in developing countries. The practical components cover media preparation, aseptic transfer of explants, and micro-propagation of plants, highlighting significant advancements in agricultural biotechnology. ###
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LECTURE 5Ø Protoplast, cell, tissue and organ culture.v In-vitro pollen germination and fertilizationv protoplasm and cell fusionv Crop propagation and somatic embryogenesis in rapid multiplication of agriculture and rural development in the developing countries.PRACTICALS:Ø Preparation of mediaØ Aseptic transfer of explantsØ Micro-propagation of plants