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Enzyme-Linked Immunosorbent Assay (Direct ELISA)

Enzyme-Linked Immunosorbent Assay (Direct ELISA). Figure 18.14a. Enzyme-Linked Immunosorbent Assay (Indirect ELISA). Figure 18.14b. Serological Tests. Figure 18.13. Serological Tests. Direct tests detect antigens (from patient sample).

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Enzyme-Linked Immunosorbent Assay (Direct ELISA)

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  1. Enzyme-Linked Immunosorbent Assay(Direct ELISA) Figure 18.14a

  2. Enzyme-Linked Immunosorbent Assay (Indirect ELISA) Figure 18.14b

  3. Serological Tests Figure 18.13

  4. Serological Tests • Direct tests detect antigens (from patient sample). • Indirect tests detect antibodies (in patient′s serum).

  5. Serological Tests • Agglutination: Particulate antigens • Hemagglutination: Agglutination of RBCs • Precipitation: Soluble antigens • Fluorescent-antibody technique: Antibodies linked to fluorescent dye. • Complement fixation: RBCs are indicator. • Neutralization: Inactivates toxin or virus. • ELISA: Peroxidase enzyme is the indicator.

  6. Question 1 • Patient’s serum, influenza virus, sheep RBCs, and anti-sheep RBCs are mixed in a tube. • Influenza virus agglutinates RBCs. • What happens if the patient has antibodies against influenza virus?

  7. Question 2 • Patient’s serum, Chlamydia, guinea pig complement, sheep RBCs, and anti-sheep RBCs are mixed in a tube. • What happens if the patient has antibodies against Chlamydia?

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