Understanding HPLC: Setup, Injector, and Column Dynamics in Liquid Chromatography

Jan 02, 2026

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This lecture provides an in-depth overview of high-performance liquid chromatography (HPLC) focusing on the setup process, injector functionality, and the significance of column dynamics. It covers concepts such as particle size, tortuosity, and the factors influencing the Van Deemter equation—key aspects for optimizing separation efficiency. Additionally, discussions on various LC column types including C8, C18, and size exclusion chromatography highlight their unique characteristics and applications. Utilize this knowledge for improved analytical results in chemical analysis.

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Understanding HPLC: Setup, Injector, and Column Dynamics in Liquid Chromatography

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Presentation Transcript

Lecture 12HPLC
Lecture 12HPLC
GC setup
LC setup
LC Injector
Back to Van Deempter A + ∞ H C
Back to Van Deempter dp2 + Ce dp ∞ H  DMP
Mobile Phase dp2 + Ce dp ∞ H  DMP
LC Columns Particle Size (dp) dp2 + Ce dp ∞ H  DMP Dong, M. Today’s Chemist at Work. 2000, 9(2), 46-48.
LC Columns Particle Size (dp) dp2 + Ce dp ∞ H  DMP
LC Columns Tortuosity (g) dp2 + Ce dp ∞ H  DMP Ascentis Express, Supelco, technical information
Injector/column interaction
Types of LC columns C8 C18
Column elution
Size exclusion chromatography