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DNA Fingerprinting & Gel Electrophoresis

DNA Fingerprinting & Gel Electrophoresis. Do Now:. DNA Fingerprinting. Everyone (except identical twins) has a unique DNA sequence in their cells. A technique called ________________________can be used to ________________________(or parents) using their DNA.

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DNA Fingerprinting & Gel Electrophoresis

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  1. DNA Fingerprinting & Gel Electrophoresis Do Now:

  2. DNA Fingerprinting • Everyone (except identical twins) has a unique DNA sequence in their cells. • A technique called ________________________can be used to ________________________(or parents) using their DNA. • DNA fingerprinting is MUCH more accurate than traditional fingerprints

  3. How DNA Fingerprinting Works • DNA is extracted from a sample (like when you got DNA from strawberries) • Special enzymes called _________________________ are used to cut the DNA samples into pieces. • The DNA pieces are then put through a process called _____________________________

  4. Gel electrophoresis • Gel electrophoresis is used to __________________________________. • DNA molecules are separated by ____________.

  5. Analyzing the Results Restriction Enzymes • The gel shown shows what happened when the same piece of DNA was cut up with 3 different enzymes. DNA fragment Standard Sizes

  6. Solving Crimes: Rape investigation • The DNA from a rapist can be collected from the victim for up to about 24 hours after the crime. • The DNA found can then be compared to the DNA of suspects. • Which suspect committed the crime?

  7. DNA Fingerprint Paternity Testing • DNA fingerprinting (with gel electrophoresis) can be used to determine who the __________ of an organism are. • Just like genes, DNA fragments are _____________ from both parents

  8. Final Thoughts • A DNA fingerprint is like a unique _______________ for every organism on Earth. • DNA fingerprinting can be used to __________________________and determine who an individual’s genetic __________________ are. • DNA fingerprinting uses ________________________to separate different size pieces of DNA created by chopping up the original piece using special enzymes

  9. mRNA Processing • Remember the central dogma of molecular biology: • ___________________________ • In __________________, there is one more little step in between RNA and protein. • After an mRNA is made by transcription, it must be __________________ before _________________.

  10. Introns & Exons • An mRNA sequence has ___________ and __________ within it. • Before being used to make proteins, the introns must be ___________________. • Intron: A part of an mRNA molecule that ____________________________ before translation. • Exon: A part of an mRNA molecule that will be _______________________________.

  11. Get rid of those Introns! • Before introns are removed, an RNA molecule is called ________________________________________ • Only the ______________ make it to translation!

  12. That’s it for Molecular Genetics • Your review guide is due tomorrow! • We will be playing Jeopardy tomorrow: your review guide will be the only notes you may use. • Use the remainder of the period (and tonight) to complete anything on the review guide you haven’t yet.

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