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Mean percentage of polarized mitochondria

Study evaluating the impact of z-VAD-FMK treatment on mitochondrial polarization and caspase activity in human neuroblastoma cells IMR-32, SH-SY5Y, HTLA-230, M14, and WM using assays for Procaspase 9, Procaspase 3, Intact PARP, Cleaved PARP, and GAPDH.

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Mean percentage of polarized mitochondria

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  1. (B) (A) GI-LI-N cells LB24 cells 30000 Control Control *** 30000 14 kDa m m D1 (10 M) D1 (10 M) *** m m D6 (5 M) D6 (5 M) *** 37 kDa z-VAD-FMK z-VAD-FMK 20000 20000 *** z-VAD-FMK+D1 z-VAD-FMK+D1 Caspase 3/7 (RFU) Caspase 3/7 (RFU) z-VAD-FMK+D6 z-VAD-FMK+D6 10000 10000 Control D1 0 0 D6 Time (24 hours) Time (24 hours) 14 kDa 37 kDa (C) (D) 100 D1 CTR D6 * * * 75 IMR-32 Mean percentage of polarized mitochondria *** GAPDH 50 Cytocrome c LB24 25 (cytosol fraction) GAPDH 0 LB24 Cytocrome c (E) IMR-32 (cytosol fraction) – + – – + – – + – – + – – + – – + – D1 – – + – – + – – + – – + – – + – – + D6 Procaspase 9 (47kD) Procaspase 3 (35kD) GAPDH (37kD) SH-SY5Y HTLA-230 LB24 M14 WM GI-LI-N Intact PARP (116kD) Cleaved PARP (85kD) GAPDH (37kD) SH-SY5Y HTLA-230 GI-LI-N LB24 M14 WM

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