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Additional File 5. A. Fold I κ B α expression (Normalized). IkB α ( 38 kDa). GAPDH (37 kDa). --. TNF α. TNF α. --. Control cells. WT-Ras cells. B. p65 (65 kDa). Total p65/GAPDH expression (Normalized). GAPDH (37 kDa). siRNA p65 (25nM). siRNA p65 (35nM). siRNA Control.

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  1. Additional File 5 A. Fold IκBαexpression (Normalized) IkBα (38 kDa) GAPDH (37 kDa) -- TNFα TNFα -- Control cells WT-Ras cells B. p65 (65 kDa) Total p65/GAPDH expression (Normalized) GAPDH (37 kDa) siRNA p65 (25nM) siRNA p65 (35nM) siRNA Control siRNAControl siRNAp65 (25nM) siRNAp65 (35nM) Control WT-Ras Additional File 5 IB levels in TNF-stimulated WT-Ras expressing cells, and p65 down-regulation by shRNAs to p65 (A) WT-Ras expressing MCF-7 cells were not-stimulated or stimulated by TNF (50 ng/ml). Activation of the NF-B pathway was analyzed by reduced levels of IB (=NF-B inhibitor), determined by WB. A representative experiment of n=3 is presented. (B) Validation of the p65-reducing activities of siRNAs to p65, determined by WB (Inhibition levels: 42% and 62% inhibition for 25 nM and 35 nM siRNA to p65, respectively). Reduction of p65 expression by siRNA p65 was denoted in n=3. -- -- TNFα TNFα

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