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Multiple Sclerosis–Associated Retrovirus (MSRV) in Sardinian MS Patients By Esther Valbrun

Multiple Sclerosis–Associated Retrovirus (MSRV) in Sardinian MS Patients By Esther Valbrun. Dolei A., G. Serra, M. Mameli, G. Pugliatti, M. Sechi, G. Cirotto, S. Rosati, and S. Sotgiu, MD . 2002. Multiple Sclerosis - associated retrovirus (MSRV) in Sardinian MS patients . Neurology 58: 471-473.

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Multiple Sclerosis–Associated Retrovirus (MSRV) in Sardinian MS Patients By Esther Valbrun

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  1. Multiple Sclerosis–Associated Retrovirus (MSRV) in Sardinian MS PatientsByEsther Valbrun Dolei A., G. Serra, M. Mameli, G. Pugliatti, M. Sechi, G. Cirotto, S. Rosati, and S. Sotgiu, MD . 2002. Multiple Sclerosis - associated retrovirus (MSRV) in Sardinian MS patients. Neurology 58: 471-473.

  2. Introduction • MSRV circulation among Sardinian MS patients • MS-associated retrovirus (MSRV) has been linked to MS pathogenesis • Multifactorial autoimmune cascade developing in MS lesion formation

  3. Objective Analyze its possible MS-specific role • Searched for MSRV Cerebralspinal fluid Blood of patients with active MS Blood of patients with other neurologic diseases (OND) Blood MSRV circulation among the general population

  4. Presence of MSRV in the CSF of patients with MS and patients with OND with or without neurologic inflammatory diseases

  5. Analysis • The CSF samples were available for 31 MS and 10 OND patients • Extracellular virus could be detected in 80.6% MS and 40% OND CSF samples

  6. Extracellular MS-associated retrovirus (MSRV) in CSF from patients with MS according to parameters related to temporal disease progression

  7. Analysis • Virus positivity was detected in 50% of CSF samples at clinical onset (figures, A and C). • Virus positivity increased according to disease duration (p = 0.009; figure A), to diagnostic level (p = 0.003; figure B), and to MS course (from onset to SP forms; p = 0.01; figure, C) • MSRV positivity already was 78% in CFS samples

  8. Presence of MSRV in the blood of patients with MS and patients with OND with or without inflammatory diseases in comparison to blood donors • Plasma MSRV was detected in 12.8% of healthy BD and in 100% of patients with MS. • 42.9% of OND patients also were MSRV positive. • 33.0% of the subjects with OND • ischemic stroke, 2; epilepsy, 1; thyrotoxicosis, 1; neurosis, 1; migraine, 1; and trigeminal neuralgia, 1 • MSRV positivity was 63.6% in the 11 OND patients with inflammatory disease • 4/5 patients with inflammatory CNS disease • 3/4 with inflammatory peripheral neuropath

  9. Discussion • MSRV positivity of OND patients with inflammatory CNS disease and peripheral neuropathy was 77.8% • MSRV presence in blood appeared to be related to the inflammatory nature of the diseases, particularly those of both peripheral and CNS origin • Observed that CSF MSRV positivity has an incremental trend being present in 100% of untreated, active SP MS patients

  10. Conclusion • 40-year span is too short a time interval for a substantial change in Sardinians’ genetic pool to occur • Sardinia is a high-risk area for MS • Detection of MSRV in MS • Detection in body fluids of patients deserves further study to elucidate pathogenic connections aimed at possible future therapeutic strategies

  11. Reference • Dolei A., G. Serra, M. Mameli, G. Pugliatti, M. Sechi, G. Cirotto, S. Rosati, and S. Sotgiu, MD . 2002. Multiple Sclerosis - associated retrovirus (MSRV) in Sardinian MS patients. Neurology 58: 471-473. • Noseworthy JH, Lucchinetti C, Rodriguez M, Weinshenker BG. Multiple sclerosis. N Engl J Med . 2000; 343: 938–962.[Full Text] • Gieffers J, Pohl D, Trieb J, et al. Presence of Chlamydia pneumoniae DNA in the cerebrospinal fluid is a common phenomenon in a variety of neurological diseases and not restricted to multiple sclerosis. Ann Neurol . 2001; 49: 585–589.[Medline] • Perron H, Garson JA, Bedin F, et al. Molecular identification of a novel retrovirus repeatedly isolated from patients with multiple sclerosis: the Collaborative Research Group on Multiple Sclerosis. Proc Natl Acad Sci USA . 1997; 94: 7583–7588.[Abstract/Full Text] • Tristem M. Identification and characterization of novel human endogenous retrovirus families by phylogenetic screening of the human genome mapping project database. J Virol . 2000; 74: 3715–3730.[Abstract/Full Text] • Garson JA, Tuke PW, Giraud P, Paranhos-Baccala G, Perron H. Detection of virion-associated MSRV-RNA in serum of patients with multiple sclerosis. Lancet . 1998; 351: 33. • Poser CM, Paty DW, Scheinberg L, et al. New diagnostic criteria for multiple sclerosis. Ann Neurol . 1983; 13: 227–231.[Medline] • Dolei A, Biolchini A, Serra C, et al. Increased replication of T-cell-tropic HIV strains and CXC-chemokine receptor-4 induction in T cells treated with macrophage inflammatory protein (MIP)-1 , MIP-1ß and RANTES ß-chemokines. AIDS . 1998; 12: 183–190.[Medline] • Pugliatti M, Sotgiu S, Solinas G, et al. Multiple sclerosis epidemiology in Sardinia: evidence for a true increasing risk. Acta Neurol Scand . 2001; 103: 20–26.[Medline] • Gaudin P, Ijaz S, Tuke PW, et al. Infrequency of detection of particle-associated MSRV/HERV-W RNA in the synovial fluid of patients with rheumatoid arthritis. Rheumatology . 2000; 39: 950–954.[Abstract/Full Text] • Menard A, Amouri R, Michel M, et al. Gliotoxicity, reverse transcriptase activity and retroviral RNA in monocyte/macrophage culture supernatants from patients with multiple sclerosis. FEBS Lett . 1997; 413: 477–485.[Medline]

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