Understanding Algal Blooms and Coliform Bacteria in Environmental Microbiology
This chapter delves into the impact of pollutants, particularly nutrients, that lead to algal blooms and subsequent eutrophication in aquatic environments. It highlights the role of coliform bacteria as indicator organisms for fecal contamination, outlining their characteristics and the methods used for detection such as the Most Probable Number (MPN) and Multiple-Tube Method. Additionally, it covers municipal water and sewage treatment processes, including activated sludge and anaerobic digestion, showcasing their importance in maintaining water quality.
Understanding Algal Blooms and Coliform Bacteria in Environmental Microbiology
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Presentation Transcript
Chapter 27, part C Environmental Microbiology
Algal Blooms • Pollutants (nutrients) may cause algal blooms. • Algal blooms lead to eutrophication. Figure 27.15
Coliforms • Aerobic or facultatively anaerobic, gram-negative, non–endospore forming rods that ferment lactose to acid + gas within 48 hr, at 35°C • Indicator organisms • Used to detect fecal contamination • MPN • Most probable number/100 ml of water
Multiple-Tube Method Figure 6.18a
Multiple-Tube Method Figure 6.18b
MUG Test - galactosidase MUG fluorescent compound • ONPG causes E. coli to make -galactosidase Figure 27.16
Municipal Water Treatment Figure 27.17
Municipal Sewage Treatment Figure 27.19
Activated Sludge Figure 27.20a,b
Anaerobic Sludge Digester • CO2 + 4 H2 CH4 + 2 H2O • CH3COOH CH4 + CO2 Figure 27.23
