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Explore the role of 18S rRNA in gene expression with luciferase and RFP assays. Investigate NcoI and KpnI restriction sites in 18S rRNA constructs to study transcription. This study delves into the influence of 18S rRNA on gene regulation.
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A) 1 2 3 4 5 6 7 8 10 6 4 18S rRNA 18S rRNA Luc RFP 18S rRNA I II R-NTS/P LUC 18S II I hyg Kpn I Kpn I 9.5 kb 18S RFP I II hyg 1.6 kb Nco I Nco I Nco I 6.0 kb B)