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海洋天然物 Elysia rufescens 海蛞蝓 Kahalalide F (KF)

海洋天然物 Elysia rufescens 海蛞蝓 Kahalalide F (KF). 指導老師：詹于誼 組員：楊仲智 張芳瑛 翁曼真. 0.2 to 4 m M using a continuous 96 hr(median = 0.75 Mm ). Images of cells treated with KF. control. KF. 0.5 m M for HepG2. 5 m M for PLC/PRF/5C. 0.1 m M. 0.5 m M.

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海洋天然物 Elysia rufescens 海蛞蝓 Kahalalide F (KF)

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  1. 海洋天然物Elysia rufescens 海蛞蝓 Kahalalide F (KF) 指導老師：詹于誼 組員：楊仲智 張芳瑛 翁曼真

  2. 0.2 to 4 mM using a continuous 96 hr(median = 0.75 Mm).

  3. Images of cells treated with KF control KF 0.5 mM for HepG2 5 mM for PLC/PRF/5C

  4. 0.1mM 0.5mM 1mM 2.5mM 5mM 0.5–0.7 mM KF in HepG2 cells KF-induced depletion of ATP in HepG2 and PLC/PRF/5C cells 1mM 2.5mM 5mM 10mM 25mM 4–6 mM KF in PLC/PRF/5C cell

  5. Analysis of KF-induced permeability changes in HepG2 cells by flow cytometry (a) control

  6. (b) treated with 0.25 mM KF for 1 h

  7. LDH release by HepG2 and PLC/PRF/5C after exposure to KF for 1 h. HepG2 PLC/PRF/5C

  8. Discussion • HepG2 cells demonstrated profound ATP depletion, associated with cell swelling and cell blebbing, and increased permeabilityto propidium iodide (PI), annexin V (AV) and release of lactate dehydrogenase (LDH).cell membrane induced by KF in HepG2 • PLC/PRF/5C cells retained their cellstructure, but were permeable to PI and, following exposure to high concentrations of KF, to AV. PLC/PRF/5C suggest specific interactions with membranes or proteins. • This could lead to better drug design aimed at exploitingthe potential for cell selectivity.

  9. Thanks for your attention

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