Download
1 / 17
180 likes | 247 Vues
APOPTOZA. Program stanične smrti može se inducirati. This shows a chicken cell undergoing apoptosis when treated with hydroxyurea. Note the dramatic morphological changes.
E N D
Program stanične smrti može se inducirati This shows a chicken cell undergoing apoptosis when treated with hydroxyurea. Note the dramatic morphological changes.
Timecourse showing a LLCPK cell undergoing apoptosis when irradiated with UV light. DNA (yellow) was stained with Hoechst 33342. Note how rapid is the collapse of the nuclear chromatin.
Sequence of time-lapse images of a LLCPK cell undergoing apoptotic cell death. Induction of apoptosis was accomplished by culturing cells in the presence of etoposide at a final concentration of 68 µM throughout observation. Green staining corresponds to fluorescence associated with Lamin A:GFP chimeric protein.
Electron micrograph of an apoptotic cell in the mammary gland. Note the dark clumps of condensed chromatin. An adjacent normal cell can be seen at the right side of the picture.
This image shows a timecourse of nuclear apoptosis. In this experiment I incubated isolated HeLa nuclei with a cytoplasmic extract prepared from cells commited to apoptosis. This extract induces apoptotic morphological changes in the HeLa nuclei.
Apoptoza apoptotičnatjelešca propidiumiodide Apoptotic human keratinocytes that were fixed with paraform-aldehyde and stained with fluorescein concanavalin A. The cells were subsequently permeabilized with acetone, stained with propidium iodide and visualized by confocal microscopy. This photomicrograph clearly shows that the green-fluorescent lectin staining outlines the apoptotic surface blebs, whereas the red-fluorescent propidium iodide stains the interior of the blebs.
fosfatidilserin Fagocit Apoptotična tijela
Kontrolirannje programirane stanične smrti u stanicama sisavaca Nature406, 135 - 136 (2000) proteins of the inhibitor-of-apoptosis (IAP) family can inhibit cell death by binding to procaspases and activated caspases, thereby blocking their processing and their activity These enzymes cleave several intracellular substrates, such as ICAD (resulting eventually in DNA fragmentation) and other cell-death regulators, ensuring the death of the cell. Verhagen et al.1 and Du et al.2 have shown that the IAP roadblock is lifted by Diablo/Smac, a protein that binds to IAPs and prevents them from inhibiting apoptosis. So Diablo/Smac determines cellular sensitivity to the apoptotic signal.
DD FADD TRADD TRADD DED TRAF FLICE TRAF2 TRAF NFkB APOPTOZA Next slide
APOPTOZA Bcl Bax Bax Bcl PREŽIVLJAVANJE
