1 / 9

Engineered reversal of the β -oxidation cycle for the synthesis of fuels and chemicals

Engineered reversal of the β -oxidation cycle for the synthesis of fuels and chemicals. 报告人：张文倩 时间： 2011.10.07. Functional reversal of the β -oxidation cycle. 前期准备：对 β -oxidation 的正调控. RB01: MG1655. fadR atoC(c) crp* Δ arcA. 脂肪酸底物不存在的情况下反应仍能进行. 去除 arcA 介导

lacy
Télécharger la présentation

Engineered reversal of the β -oxidation cycle for the synthesis of fuels and chemicals

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Engineered reversal of the β-oxidation cycle for thesynthesis of fuels and chemicals 报告人：张文倩 时间： 2011.10.07

  2. Functional reversal of the β-oxidation cycle

  3. 前期准备：对β-oxidation的正调控 RB01: MG1655 fadR atoC(c) crp* ΔarcA 脂肪酸底物不存在的情况下反应仍能进行 去除arcA介导 对β-氧化的抑制作用 使之独立于cAMP RB02: MG1655 fadR atoC(c) crp* ΔarcA ΔadhEΔptaΔfrdA: 封锁其他代谢途径 产乙醇 产乙酸 产琥珀酸

  4. 针对一轮反应，以n-butanol的合成为目标，调节各步反应的基因针对一轮反应，以n-butanol的合成为目标，调节各步反应的基因 • 终止反应： YqhD FucO两个基因分别过表达 Butanol 产量升高 • 第一步 ： acetyl-CoA to acetoacetyl-CoA [atoB+] and [yqeF+] Butanol 产量升高 • 副产物ethanol的产量减少： ΔeutE ΔYqhD

  5. 总结各步反应 （1） YqeF （2） FadB （3） FadB （4） YdiO （5） MhpF and FucO

  6. 第四步：β-氧化中唯一不可逆反应 YdiO FadE ④ • Ydio 替换 FadE A new Escherichia coli metabolic competency: growth on fatty acids by a novel anaerobic β-oxidation pathway. Mol.Microbiol. 47, 793–805 (2003). • 热力学可行性分析： YdiQRST 编码 ferredoxin and flavoproteins ferredoxin 作 enoyl-CoA to acyl-CoA 的还原力ΔG﹤0 • 结论： Ydio –YdiQRST 用于enoyl-CoA to acyl-CoA此步还原反应 逆反应 ③ FadB FadB ② ① YqeF

  7. 最后，与其他合成途径做对比 Malonyl-ACP的合成消耗 ATP acyl-ACP intermediates消耗ATP fatty-acid biosynthesis a nonnatural metabolic engineering approach 目前合成最长碳链水平：己醇 50% C-mole keto-acid pathways 高效性 reversal of the β-oxidation cycle 66.7%C-molebasis 每加两个C原子单元，生成一个ATP 直接以acetyl-CoA作碳原子供体 acyl-CoA intermediates作为n-alcohols和其他产物的前体

  8. Thank you!

More Related