1 / 3

Immunohistochemical Analysis of Rabies and HIV Co-infection in Neuronal Cells

This slide illustrates a rabies-infected neuronal cell exhibiting intracytoplasmic inclusions. The red staining highlights regions containing rabies viral antigens, through the use of immunohistochemistry (IHC) with the avidin-biotin complex (ABC) technique. Additionally, confocal microscopy reveals double immunofluorescent staining for RCA-1 (red) and HIV gp41 protein (green), with evidence of HIV co-infection indicated by the yellow co-localization with RCA-1-stained macrophages. An immunofluorescence assay demonstrates SARS-CoV-infected Vero cells treated with patient serum post-symptom onset.

lalasa
Télécharger la présentation

Immunohistochemical Analysis of Rabies and HIV Co-infection in Neuronal Cells

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. This slide shows a rabies-infected neuronal cell with intracytoplasmic inclusions. The red stain indicates areas of rabies viral antigen by using IHC or avidin-biotin complex (ABC) technique.

  2. Confocal microscopy of double immunofluorescent staining for RCA-1 (red) and HIV gp41 protein (green). At least one of perivascular RCA-1 stained macrophages is infected with HIV, as evidenced by co-localization of RCA-1 and gp41 (yellow).

  3. Immunofluorescence assay (IFA): SARS-CoV-infected Vero cells incubated with patient serum (1:50 dilution) obtained 11 days after the onset of symptoms, showing cytoplasmatic fluorescence. (Source: Institute for Medical Virology, Director: W. Doerr)

More Related