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This study investigates the nDart distribution across 63 accessions of the rice core collection and 38 accessions from the Japanese rice core collection. Genomic DNA was digested with DraI and analyzed through electrophoresis on 0.6% agarose gels. The DNA was subsequently blotted onto a filter and hybridized with a probe consisting of the 309-bp EaeI fragment of nDart. Results displayed subspecies classifications: japonica (J), aus (A), and indica (I), with lane numbers referenced in Tables S1 and S2.
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Fig. S1 nDart distribution among cultivated ricevarieties, a total of 63 accessions of the rice core collection. Genomic DNA was digested with DraI. After electrophoresis in 0.6% agarose gels, DNA was blotted on to a filter and hybridized with a probe comprised of the 309-bp EaeI fragment of nDart. M indicates size marker of HindIII-digested lamda DNA. Numbers indicate the lane numbers listed in Table S1. J (japonica), A (aus), and I (indica) indicate the subspecies classification of cultivated rice.
Fig. S2 nDart distribution among cultivated rice varieties, a total of 38 accessions of the Japanese rice core collection. Genomic DNA was digested with DraI. After electrophoresis in 0.6% agarose gels, DNA was blotted on to a filter and hybridized with a probe comprised of the 309-bp EaeI fragment of nDart. M indicates size marker of HindIII-digested lamda DNA. Numbers indicate the lane numbers listed in Table S2.