Is your drinking water safe?

1. Is your drinking water safe? Please check with “Drinking Water HometestSeries” Drinking Water Hometest suggest simple and easy drinking water safety monitoring by self-conducting bacterial test in home. The protocol is very simple and the principle, color change of water sample induced by bacteria’s specific enzyme reaction, is recommended as official test method for water safety test in many countries. High reliability Rapid detection Safe wasting Easy reading Simple ▶Specific enzyme reaction coupled with color change! (for Coliforms & E. coli) ▶Easy protocol and convenient testing! ●Even if you are not a scientist, you can test bacterial safety of water sample by yourself with our “Drinking Water Hometest” because the protocol is very simple and everything’s prepared in kits. ●You can read the result within 18 hrs to 24 hrs. ●You can achieve reliable test result with our principle, so called “color change of water sample induced by bacteria-specific enzyme reaction” is recommended as official test method for water safety test in many countries. ●It is so sensitive and reliable as even one bacterium is detected. ●We provide safe wasting of the test materials with Bacterzero, natural-origin bacteria sterilization reagent. After testing add Bacterzero into your water sample and prohibit secondary contamination. BacterView ··································· Detection of total aerobes ColiView E.ColiView ··································· ··································· Detection of coliforms and E. coli simultaneously Detection of coliforms

2. Drinking Water Hometest BacterView for total aerobes BacterView is a kit detecting total aerobic bacteria in your drinking water, which change the color to red. Total aerobic bacteria indicate contamination or stability of water and exist widely in river, ground water, soil, foods, air and so on. They grow in the presence of oxygen and too many numbers can cause stomach disorder or diarrhea. Specification Triphenyl- tetrazolium chloride Formazan Reduction by Dehydrogenase Principle Redox dye (2,3,5-triphenyl tetrazolium chloride, TTC) is converted to formazan because of reduction by dehydrogenase of bacteria, which indicates red color. Test Procedure 1 2 3 4 5 Add 20 mL sample to a medium tube with a filled volume of 4 mL. Add 1 mL substrate to the medium tubefilled with sample plus medium. Incubate the sample with medium at 35~37℃ for 16~24 hrs. (at ambient temp. for 36 hrs.) Read the result Add 2 mL of BacterZero to the test-finished tube and mix. Leave for 30 min. and discard it insideinto a toilet. Interpretation of Result Storage condition and Shelf life If aerobic bacteria exist in your drinking water, the color of the medium will be changed to red slowly. The color concentration depends on the number of aerobic bacteria. ●Dark place, 4~25℃ ● 6 months after manufacture Negative for total aerobes Positive for total aerobes

3. Drinking Water Hometest ColiView for coliforms Coliview is a kit testing presence and absence of coliforms in your drinking water allows specific enzyme-chromogenic substrate reaction. Coliforms are important indicators of fecal contamination. If coliform bacteria exist (blue color), you should enhance hygiene practice because the result provide a possibility of other food poisoning pathogens. Specification Principle If coliforms are present, they use the enzyme β-galactosidase (produced by all coliforms, including E.coli) to metabolize the specific color-producing substrate, X-gal and the medium changes color from near colorless to blue. Test Procedure 1 2 3 4 5 Add 20 mL sample to a medium tube with a filled volume of 4 mL. Add 1 mL substrate to the medium tubefilled with sample plus medium. Incubate the sample with medium at 35~37℃ for 16~24 hrs. (at ambient temp. for 36 hrs.) Read the result Add 2 mL of BacterZero to the test-finished tube and mix. Leave for 30 min. and discard it insideinto a toilet. Interpretation of Result After incubation, the color of medium in reaction bottle would be changed to blue under presence of total coliforms in sample water. On the other hand, color of medium would not be changed under absence of total coliforms in sample. Storage condition and Shelf life X-gal ●Dark place, 4~25℃ ● 6 months after manufacture Negative for coliforms Positive for coliforms β-galactosidase

4. Drinking Water Hometest E.ColiView for coliforms & E. coli E.Coliview use specific enzyme-chromogenic substrate reaction to simultaneously detect total coliforms and E.coli from home and environmental water samples. Coliforms including E. coli are important indicators of fecal contamination. If they exist (blue color) in your drinking water, you should enhance hygiene practice of it because the result provide a possibility of other food poisoning pathogens. Specification Principle If coliforms are present, they use the enzyme β-galactosidase to metabolize X-gal and the medium changes color from near colorless to blue. If E.coli are present, they use β-glucuronidase (produced by E.coli strains but not by general coliforms) to metabolize MUG and create fluorescence. Test Procedure 1 2 3 4 5 Add 20 mL sample to a medium tube with a filled volume of 4 mL. Add 1 mL substrate to the medium tubefilled with sample plus medium. Incubate the sample with medium at 35~37℃ for 16~24 hrs. (at ambient temp. for 36 hrs.) Add 2 mL of BacterZero to the test-finished tube and mix. Leave for 30 min. and discard it insideinto a toilet. Read the result Interpretation of Result Coliform-positive reaction turns the medium blue. The blue color means that total coliforms are present in the sample. If no color change means that there are no presence of total coliforms in the test sample. E. coli-positive reaction caused the medium to fluorescence under a long-wavelength UV lamp (365nm). If a bright blue fluorescence is observed, this indicates that MUG-positive E. coli bacteria are present. Storage condition and Shelf life X-gal MUG ●Dark place, 4~25℃ ●6 months after manufacture Negative for coliforms Positive for coliforms Positive for E. coli β-galactosidase β-glucuronidase

5. Product Description ●In Vitro Diagnostic Kit, 2 Tests. ●Keep out of reach of children so that they might not eat. ● Keep storage conditions and use the product within the expiry date. We cannot guarantee the performance if you do not follow the instructions. (Date of manufacture is indicated on a vacuum packed plastic bag) ● Be sure to dispose using BacterZero after testing. ※ For your reference! Excellent bactericidal Effect of BacterZero ●Escherichia coli ●Bacillus cereus (Spore-forming bacterium) Control (No BacterZero) Control (No BacterZero) 10-3 10-4 10-5 Dilution Rate Dilution Rate 10-3 10-4 10-5 Test (Addition of BacterZero) Test (Addition of BacterZero) 5 min.20min.60 min. 5 min.20min.60 min. Time Time