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Saving the Ozark Chinquapin

Restoring the Ozark chinquapin by utilizing natural genetic resistance

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Saving the Ozark Chinquapin

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  1. One of the most important defense mechanisms to a disease caused by a fungus is genetic resistance

  2. Evolution is natures own breeding program Since oc rarely reproduce in the wild there is little chance for them to evolve on their own We are helping along a process that would take 100s years to happen on its own in nature Major benefit: allows pathogen and host to continue to co-evolve

  3. Host resistance in is not controlled by a single gene Resistance is quantitative, controlled by a combination of genes

  4. There is lot of genetic diversity in oc Openly pollinated High genetic diversity is key, we can capture some of the rare alleles for resistance Show pictures

  5. In 2007 when the ocf started, we started selecting oc trees for our restoration breeding program (hundreds of trees, 12 test plots) (location) Goal: conserving genetic diversity Parent trees , crosses, and grafts All 100% pure OC

  6. Allowed to openly pollinate All the trees produce thousands of seed every year No blight so far

  7. Partnering with the public, federal, state & local authorities to bring back the OC Ozarkchinquapin.com Since 2008 we have mailed tens of thousands of seed from research plots for outplanting across the native range -database tracks gen lines -

  8. Partnership with mobot

  9. Screening for resistance Detatched leaf assay, rapid screening for evaluating for blight resistance -in ten days evaluate ten years of work -involves detaching leave from tree, inoculating leaves then measuring the resulting necrosis visible on the leaf -Does not harm host tree -i can test tree of any age, seedling or a hundred years old

  10. Leaf assay methods Method developed by Andy Newhouse for chestnuts

  11. Isolating chestnut blight Preparing innoculum Bark samples were taken from the leading edge of cankers on infected OC, were surface sterilized and plated on agar to grow

  12. Isolating chestnut blight Preparing innoculum After isolating c. parasitica, cultures were transferred to new plates and maintained on PDA Subculture 4-5 days before inoculation 5mm a day in culture

  13. Controls used in the study

  14. Selecting and Collecting

  15. Analyzing data

  16. Results

  17. Chestnut blight Chestnut blight is a non-native fungal pathogen caused by cryphonectria parasitica

  18. Chestnut blight -Necrotroph It enters wounds in the tree, colonizes, and forms mycilial fans in the cambium layer under the bark.

  19. Chestnut blight

  20. Chestnut blight

  21. Road to Restoration

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