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Understanding Class II Promoters, Enhancers, and Silencers in Molecular Genetics

This lecture delves into the intricacies of Class II promoters, particularly focusing on RNA polymerase II, along with the roles of enhancers and silencers. Key elements such as the TATA box and GC box are explored, highlighting their functions and importance in positioning transcription start sites. The lecture also reviews Class I and Class III promoters, emphasizing their structural variations and specific functions in transcription of rRNA and tRNA genes. Visual aids including figures illustrate the concepts of linker scanning and transcription dynamics in a detailed and engaging manner.

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Understanding Class II Promoters, Enhancers, and Silencers in Molecular Genetics

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  1. Molecular Genetics Bio350/516 Lecture 17, 3/28/06 I. Chapter 10 (continued) A. Promoters 1. Pol II (class II) a. Linker scanning 2. Pol I (class I) 3. Pol III (class III) B. Enhancers and Silencers

  2. Class II Promoters: mRNA genes

  3. Figure 10.20 Composite of a Class II promoter: Not all elements are in all class II promoters

  4. TATA Box Function

  5. Figure 10.21 Role of the TATA box in positioning the start site

  6. Discovery of GC Box Elements

  7. Linker-Scanning

  8. Figure 10.22

  9. Frog Oocytes Diameter of 1.0 – 1.2 mm

  10. GC box TATA box GC box 23

  11. Class I Promoters: rRNA genes

  12. Figure 10.24 Linker-Scanning of human rRNA gene TXN = Transcription

  13. Figure 10.25

  14. Class III Promoters: 5S rRNA & tRNA genes (classical) (promoters are internal) nonclassical genes (some look like Class II promoters)

  15. Figure 10.26

  16. Figure 10.27a

  17. Figure 10.27b

  18. Figure 10.28

  19. Enhancers

  20. Figure 10.30

  21. Figure 10.31a Intron Exons Exons X = XbaI site

  22. Figure 10.31b Protein Levels

  23. Figure 10.31c Transcript Levels

  24. Figure 10.32 Orientation and Position Independent Enhancer

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