Advanced Topics in ChBE

1. Advanced Topics in ChBE Materials and Interfaces Biomolecular Engineering Environmental Engineering Micro and Nano Fabrication

2. Molecular Bioengineering Molecular Bioengineering exists at the interface between engineering and molecular biology (cells and molecules) and focuses on both understanding and engineering complex living systems for applications ranging from drug delivery and tissue engineering to biological synthesis of alternative fuels. ΔT Kevin Ling ‘12 q q Co-localization of focal adhesion complexes in fibroblasts cultured on thermoresponsive polymer brushes.

3. Materials & Interfaces Frequency, pulse width, voltage Microdrop NI-IMAQ Image Acquisition A fundamental understanding of the physical and chemical properties of interfaces in natural and engineered materials is of paramount importance and finds engineering significance in fields as diverse as drug delivery, water treatment, semiconductor processing, biology, and nanotechnology. Voltage generator+ Oscilloscope binder reservoir strobe CCD Camera Monitor

4. Environmental Engineering While chemical engineers are trained to deal with all aspects of environmental issues, their main focus has been in air pollution control, solid waste management, and hazardous waste management. In response to the growing demand for energy and adverse environmental impacts of fossil fuels, chemical engineers have been active in search for new fuel sources including conversion of waste materials (plastics, cellulosic compounds, etc.) and production of diesel fuel from algae. Adhesion and Cohesion in E. coli Mutant Biofilms Environmental Fate and Transport of MS2 Bacteriophage Alternative Energy Production from Sustainable Sources

5. Micro-and Nano Fabrication Chemical engineers are also increasingly applying their fundamental knowledge of chemistry, physics, and math to “scale-down” processes, thereby allowing for a reduction in material and spatial requirements while providing for more controlled operating conditions. This scale-down gives rise to the need to fabricate systems that span length scales that can be on the order of microns to nanometers.

6. Advanced Topics in ChBE Materials and Interfaces Biomolecular Engineering Environmental Engineering Micro and Nano Fabrication

7. Lauren S. Anderson Assistant Professor B.S. Chemical Engineering, Lafayette College Ph.D. Biomedical Engineering, University of Virginia Cells communicate dynamically with their environment DNA  RNA  PROTEIN Nucleus ? Research Area: Cell-material Interactions How does cell phenotype (behavior) change when a cell is cultured on a different biomaterials? Or rather, Can cell phenotype be controlled by altering the substrate? Substrate

8. Project #1: Thermoresponsive Polymers LCST: 32oC LCST: tunable, 26oC- 90oC LCST: Lower Critical Solution Temperature PNIPAM P(MEO2MA-co-OEGMA)  “PMO” • Below LCST  polymer miscibility • Above LCST  phase separation

9. Vary LCST, quantify cell phenotype 37o C: ABOVE LCST 25o C: BELOW LCST Hydrophobic  Cell adhesion Hydrophilic  Cell detachment Use tools from Molecular Biology: Real-time RT-PCR (gene expression), microscopy (phase and confocal), Western blots/ELISA (protein expression) to quantify cellular phenotype

10. Project #2: Electrospinning Scaffold architecture influences cellular phenotype Native Extracellular Matrix Science 2005, 310, (5751), 1135-1138

11. d Mimic ECM by electrospinning

12. (Nano)particle Colloidal and Interfacial Phenomena: Bioactive Materials (II) (I) (III) DW Suspending Medium Solid-liquid interface + S k11 Aggregated Nanoparticles Biomacromolecule Nanoparticle ROS Fy Nano-bio interface Biomembrane mimic Ag KO/W t cell not to scale Fx

13. James K. Ferri Associate Professor B.S. Johns Hopkins University Ph.D. Johns Hopkins University • Research Interests: • Stability in disperse systems • Manufacturing and materials processing in microgravity, • Nanocomposites and bioactive thin film mechanics

14. Nanomechanicsand Interfacial Stabilization Surfactants Polymers Particles

15. (Nano)particle Colloidal and Interfacial Phenomena Adsorption Dynamics and Interfacial Rheology + DT Aggregation Kinetics +

16. (Nano)particle Colloidal and Interfacial Phenomena Adsorption Dynamics and Interfacial Rheology + DT Aggregation Kinetics +

17. Summary I, wisdom, dwell with prudence and find out knowledge of witty inventions. Proverbs 8.12

18. JavadTavakoli • AEC 229; tavakoli@lafayette.edu ; (610) 330-5433 • Ph.D., New Jersey Institute of Technology; Newark, NJ • M.S., Illinois Institute of Technology, Chicago, Ill • B.S., Shiraz University, Shiraz, Iran • P.E., Pennsylvania • Teaching areas: kinetics and reactor design, unit operations, chemical engineering laboratories, environmental engineering, alternative energy sources

19. Javad Tavakoli, Ph.D., P.E. Professor B.S. Shiraz University, M.S. Illinois Institute of Technology Ph.D. New Jersey Institute of Technology • Renewable energy sources • Biomass to fuel • Waste to fuel • Catalysis • Sustainability • Industrial wastewater treatment • Hazardous waste treatment

20. Current Research Projects • Conversion of algae to fuel • Conversion of waste plastics to fuel • Catalytic conversion of methanol to higher oxygenates • ‘Sustainability’ and higher ed. institutions

21. Polly R. Piergiovanni, Ph.D. Assistant Professor B.S. Chemical Engineering, University of Kansas Ph.D. Chemical Engineering, University of Houston Biofuel from algae with Prof. Tavakoli Kinetics of Dye Adsorption

22. Joshua A. Levinson, Ph.D. Assistant Professor Dept. of Chemical & Biomolecular Engineering • Research Areas: • Semiconductor processing • technology • Microfluidics • Chemical kinetics • Transport phenomena • Micro- and Nano-Fabrication Lab: • Photolithography • Soft-lithography • Microscopy • Microfluidics Lab Equipment: • Also: • Inverted microscope w/ • digital imaging • PDMS prep/oven • Hot Plates • Syringe pumps • Ellipsometer • Disposables Plasma Chamber Spin Coater UV Exposer

23. Modeling and Simulation of Hydrogen Diffusion and Impurity Passivation in Zn-doped InP • Theoreticalmodels for transport and kinetics of hydrogen in III-V semiconductors • Density of states, field-enhanced diffusion,reversible reaction kinetics, etc. • Goal: Validate a predictive computersimulation • Experimental work derived from literature,prior work, AND through collaboration • Theoretical work via derivation andcomputational software (e.g., MATLAB) • Microfluidics • Microfluidics deals with the behavior, precise control, and manipulation of fluids that are geometrically constrained to a small scale (sub-millimeter or less) • Significant advantages for processing and for process development • Initial work focusing on droplet formation and droplet emulsions

24. Research Opportunities • Fundamental and applied problems involving chemical kinetics and transport phenomena • Semiconductor processing • Dopantpassivation in III-V materials - modeling and computation • Other topics possible (e.g., etching, growth, etc.) • Microfluidics studies • Droplet formation, emulsions, and dynamics • Reactions • Potential for drug delivery and lab-on-chip applications • Projects via EXCEL, Honors Thesis, and Independent Study formats