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ARDRA Identification Scheme for Coryneforms via 16S rDNA Restriction Patterns

This article presents a detailed analysis of ARDRA (Amplified Ribosomal DNA Restriction Analysis) patterns obtained from the restriction of amplified 16S rDNA using various restriction enzymes (CfoI, AluI, and RsaI). The study categorizes different Corynebacterium species and related coryneform groups based on their unique restriction patterns, facilitating identification. The presented figures showcase distinct patterns for species including *Corynebacterium afermentans, C. diphtheriae,* and various coryneform groups, enhancing the understanding of their genetic diversity.

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ARDRA Identification Scheme for Coryneforms via 16S rDNA Restriction Patterns

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  2. Lanes indicated with M: molecular size markers (100 base pair ladder (Pharmacia Biotech, Uppsala, Sweden). Figure 1. ARDRA patterns obtained after restriction of the amplified 16S rDNA with CfoI. 1: pattern 1, Corynebacterium afermentans subspecies afermentans (CIP 103499T) 2: pattern 2, CDC coryneform group ANF 1-like (IBS T30014) 3: pattern 3, C. minutissimum (ATCC 10288T) 4: pattern 4, C. urealyticum (ATCC 43042T) 5: pattern 4+, CDC coryneform group ANF 3-like (IBS T88848) 6: pattern 5, C. amycolatum (CIP 103452) 7: pattern 6, C. pseudotuberculosis bv. equi (CIP 5297) 8: pattern 7, CDC coryneform group B-1 (IBS T94631) 9: pattern 8, CDC coryneform group B-3 (IBS T95303) (Pattern 9 (coryneform group B-3 (IBS T79396) : not shown) Figure 2. ARDRA patterns obtained after restriction of the amplified 16S rDNA with AluI. Lane numbers correspond with pattern numbers: 1: Corynebacterium afermentans subspecies afermentans (CIP 103499T) 2: C. afermentans subsp. lipophilum (CIP 103500T) 3: C. diphtheriae (CIP 100721T) 4: C. jeikeium (CIP 103337T) 5: C. pilosum (ATCC 29592T) 6: C. propinquum (CIP 103792T) 7: C. cystitidis (CIP 6940) 8: 'C. macginleyi' (IBS B72932) 9: CDC coryneform group F-1, genomospecies 1 (IBS B1657) 10: C. pseudotuberculosis bv. ovis (CIP 102968T) 11: C. renale (CIP 6937) 12: CDC coryneform group ANF3-like (IBS T88848) 13: CDC coryneform group B-1 (IBS T94631) 14: CDC coryneform group B-3 (IBS T79396) 15: Rhodococcus equi (CIP 5472T). Figure 3. ARDRA patterns obtained after restriction of the amplified 16S rDNA with RsaI. 1: pattern 1, C. afermentans subsp. lipophilum (CIP 103500T) 2: pattern 2a, C. diphtheriae (CIP A 102) 3: pattern 2b, C. pseudotuberculosis bv. equi (CIP 5297) 4: pattern 2c, C. pseudotuberculosis bv. ovis (CIP 102968T) 5: pattern 3, C. propinquum (IBS B50121) 6: pattern 4, C. striatum (CDC E 8873) 7: pattern 5, C. urealyticum (ATCC 43043) 8: pattern 6, C. xerosis (IAM 12431) 9: pattern 7, CDC coryneform group G, genomospecies 1 (LCDC 81613) 10: pattern 8, CDC coryneform group F-1, genomospecies 1 (IBS B1657) 11: pattern 9, CDC coryneform group ANF 3-like (IBS T88848) 12: pattern 10, C. renale (CIP 103421T) 13: pattern 11, CDC coryneform group B-3 (IBS T95303) 14: pattern 12, CDC coryneform group B-3 (IBS T79396) 15: pattern 13, Rhodococcus equi (CIP 5472T)

  3. ARDRA identification scheme of coryneforms

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