Dose-Dependent Inhibition of Cytokine Production by Sonicated S. mitis in PBMCs
This study investigates the impact of sonicated S. mitis on cytokine production in peripheral blood mononuclear cells (PBMCs) from six blood donors. PBMCs were stimulated with intact S. mitis at a concentration of 3×10^7/ml, and various doses of sonicated bacteria were added. The cytokines TNF, IL-12, IL-6, IL-8, and IL-10 were measured in the supernatant after 24 hours, while IFN-γ was assessed after 5 days. Results indicate a dose-dependent inhibition of these cytokines, highlighting the immunomodulatory effects of sonicated S. mitis.
Dose-Dependent Inhibition of Cytokine Production by Sonicated S. mitis in PBMCs
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Presentation Transcript
* IFN-γ IL-12 TNF * * * * 40 1000 3 30 750 2 ng/ml ng/ml ng/ml 20 500 1 10 250 0 0 0 + sonic3×107/ml + sonic3×106/ml + sonic3×105/ml + sonic3×107/ml + sonic3×106/ml + sonic3×105/ml + sonic3×107/ml + sonic3×106/ml + sonic3×105/ml S. mitis 3×107/ml S. mitis 3×107/ml S. mitis 3×107/ml * 100 100 75 75 IL-6 IL-8 IL-10 4 50 50 3 25 25 ng/ml ng/ml ng/ml 2 0 0 1 0 + sonic3×107/ml + sonic3×106/ml + sonic3×105/ml + sonic3×107/ml + sonic3×106/ml + sonic3×105/ml + sonic3×107/ml + sonic3×106/ml + sonic3×105/ml S. mitis 3×107/ml S. mitis 3×107/ml S. mitis 3×107/ml FIG. S4. FIG. S4. Sonicated S. mitis dose-dependently inhibit the production of TNF, IL-12 and IFN-γ in response to intact S. mitis. PBMC from six blood donors were stimulated with 3 × 107 bacteria/ml of S. mitis CCUG 31661 and graded concentrations of sonicates of the same bacteria were added. Cytokines were measured in the supernatant after 24 h (TNF, IL-12, IL-6, IL-8 and IL-10) or 5 d (IFN-γ). Medians and quartiles are shown by boxes and the bars represent the 10th and 90th percentiles, respectively. The response to intact bacteria with and without addition of sonicated bacteria was compared using Wilcoxon matched pairs test. * p < 0.05.
