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ISO Documentation on Antimicrobial Susceptibility Testing Against Aerobic Bacteria

This document outlines the ISO standard for susceptibility testing of infectious agents, detailing the reference method for evaluating the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria. It specifies procedures for preparing test organisms using cation-supplemented Mueller-Hinton broth and incubation conditions. The document also defines "Intermediate" susceptibility and lists reference bacterial strains, including Staphylococcus aureus and Escherichia coli, providing essential guidelines for accurate antimicrobial testing and interpretation of results.

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ISO Documentation on Antimicrobial Susceptibility Testing Against Aerobic Bacteria

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  1. Nyt ISO dokument • Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices. • Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases.

  2. ISO reference metode • Mikrotitter bouillon fortynding • Cation supplementeret Mueller Hinton bouillon • Inokulat: 5 x 105 CFU/ml (2-8 x 105 CFU/ml) • Inkubations tp: 34-37C, atmosfærisk luft, 18 ± 2 timer.

  3. Intermediate I • bacterial strain that is inhibited in vitro by a concentration of an antimicrobial agent that is associated with uncertain therapeutic effect • NOTE: This class of susceptibility implies that an infection due to the isolate may be appropriately treated in body sites where the drugs are physiologically concentrated or when a high dosage of drug can be used. • NOTE: This class also indicates a “buffer zone” that should prevent small, uncontrolled, technical factors from causing major discrepancies in interpretations.

  4. ISO reference isolater • Staphylococcus aureus ATCC 29213 (NCTC 12973, CIP 103429, DSM 2569) • Enterococcus faecalis ATCC 29212 (NCTC 12697, CIP 103429, DSM 2570) • Escherichia coli ATCC 25922 (NCTC 12241, CIP 7624, DSM 1103) • Pseudomonas aeruginosa ATCC 27853 (NCTC 12973, CIP 54127, DSM 1117) • Streptococcus pneumoniae, ATCC 49619 (NCTC 12977) • Escherichia coli ATCC 35218 (DSM 5564)

  5. ISO reference værdier • Identiske med kendte CLSI / Eucast værdier

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