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Assay for an Antibiotic Resistance Enzyme

Assay for an Antibiotic Resistance Enzyme. Carolina Kit. Timeline. Tuesday—Lecture,Review concepts of procedure Thursday—Part 1 Part 2 (volunteer to serve as demonstrator—4 hours lab service) Monday--handouts due. Prep. For lab—Assay for Anti-biotic resistance. 2 days prior (Tuesday)

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Assay for an Antibiotic Resistance Enzyme

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  1. Assay for an Antibiotic Resistance Enzyme Carolina Kit

  2. Timeline Tuesday—Lecture,Review concepts of procedure Thursday—Part 1 Part 2 (volunteer to serve as demonstrator—4 hours lab service) Monday--handouts due

  3. Prep. For lab—Assay for Anti-biotic resistance 2 days prior (Tuesday) --label tubes control (8--15mL tubes) pAMP (8--15 mL tubes) penicillin-G (8--15 mL tubes) --streak fresh plate of MM294 and MM294/pAMP (24 hours at 37 incubator) 1 day prior (Wednesday) --prepare cultures (1/group)—see teacher guide (24 hours at 37 incubator) --penicillin-G solution (store at -20 until use) --aliquot (8 groups) • MM294 5ml overnight culture, label control • MM294/pAMP 5ml overnight culture, label pAMP • 5ml of 4mg/mL penicillin-G On lab day 1 (Thursday) --test the reagents with phenol red Lab day 2 (Monday) --demonstrator of spectrometer

  4. Write-up • Complete all aspects of the handout (50 points) Staple these together. Drawings should be neat!

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